Fluorescence observation system and set of filters
A fluorescence observation system, a method for performing a fluorescence observation, and a set of filters that can be used in such system and method are provided.
1. A set of filters comprising an illumination light filter and an observation light filter;
wherein a transmission characteristic of the illumination light filter is a sum of a first partial characteristic and a second partial characteristic;
wherein a transmission characteristic of the observation light filter comprises at least one third partial characteristic;
wherein the first partial characteristic has, at wavelengths below a threshold wavelength, at least one wavelength range in which the transmission has values greater than a first value;
wherein the second partial characteristic has, at wavelengths above the threshold wavelength, at least one second wavelength range in which the transmission has values less than a second value and greater than a third value, wherein the transmission of the illumination light filter has, between the first wavelength range and the second wavelength range, values less than a fourth value;
wherein the third partial characteristic has, at wavelengths above the threshold wavelength, at least one third wavelength range in which the transmission has values greater than the first value; and
wherein the fourth value is less than the third value, the third value is less than the second value and the second value is less than the first value,
wherein:
∫
S
T
L
(
r
→
)
·
T
O
(
r
→
)
·
r
→
·
ⅆ
r
∫
S
T
L
(
r
→
)
·
T
O
(
r
→
)
·
ⅆ
r
=
R
→
and
W
→
-
R
→
≤
0.15
;
wherein
{right arrow over (r)} is a coordinate in the CIE xy chromaticity diagram of the CIE 1931 XYZ color space,
S is a line called the spectral locus line of the CIE xy chromaticity diagram of the CIE 1931 XYZ color space, and
{right arrow over (W)} is the white point in the CIE xy chromaticity diagram of the CIE 1931 XYZ color space.
2. A set of filters comprising an illumination light filter and an observation light filter;
wherein a transmission characteristic of the observation light filter has, at wavelengths below a threshold wavelength, at least one wavelength range in which a transmission has values which are greater than a first value, and the transmission characteristic of the illumination light filter has, at wavelengths above the threshold wavelength, at least one wavelength range in which the transmission has values which are less than a fourth value;
wherein a transmission characteristic of the observation light filter has, at wavelengths above the threshold wavelength, at least one wavelength range in which the transmission has values greater than the first value, and the transmission characteristic of the observation light filter has, below the threshold wavelength, at least one wavelength range in which the transmission has values which are smaller than the fourth value;
wherein
∫
S
T
L
(
r
→
)
·
T
O
(
r
→
)
·
r
→
·
ⅆ
r
∫
S
T
L
(
r
→
)
·
T
O
(
r
→
)
·
ⅆ
r
=
R
→
and
W
→
-
R
→
≤
0.15
;
wherein:
λ designates the wavelength,
T L (λ) is the transmission characteristic of the illumination light filter,
T O (λ) is the transmission characteristic of the observation light filter, and
{right arrow over (r)} is a coordinate in the CIE xy chromaticity diagram of the CIE 1931 XYZ color space,
S is a line called the spectral locus in the CIE xy chromaticity diagram of the CIE 1931 XYZ color space, and
{right arrow over (W)} is the white point in the CIE xy chromaticity diagram of the CIE 1931 XYZ color space.
3. The set of filters according to claim 2 , wherein
|{right arrow over (W)}−{right arrow over (R)}|≦0.1.
4. A fluorescence observation system comprising:
a light source for illuminating an object;
observation optics for imaging the object;
an illumination light filter disposed in an illumination beam path between the light source and the object; and
an observation light filter disposed in a beam path of the observation optics;
wherein a transmission characteristic of the observation light filter has, at wavelengths below a threshold wavelength, at least one wavelength range in which a transmission has values which are greater than a first value, and the transmission characteristic of the illumination light filter has, at wavelengths above the threshold wavelength, at least one wavelength range in which the transmission has values which are less than a fourth value;
wherein a transmission characteristic of the observation light filter has, at wavelengths above the threshold wavelength, at least one wavelength range in which the transmission has values greater than the first value, and the transmission characteristic of the observation light filter has, below the threshold wavelength, at least one wavelength range in which the transmission has values which are smaller than the fourth value; and
wherein:
∫
S
T
L
(
r
→
)
·
T
O
(
r
→
)
·
r
→
·
ⅆ
r
∫
S
T
L
(
r
→
)
·
T
O
(
r
→
)
·
ⅆ
r
=
R
→
and
W
→
-
R
→
≤
0.15
;
wherein:
λ designates the wavelength,
T L (λ) is the transmission characteristic of the illumination light filter,
T O (λ) is the transmission characteristic of the observation light filter, and
{right arrow over (r)} is a coordinate in the CIE xy chromaticity diagram of the CIE 1931 XYZ color space,
S is a line called the spectral locus in the CIE xy chromaticity diagram of the CIE 1931 XYZ color space, and
{right arrow over (W)} is the white point in the CIE xy chromaticity diagram of the CIE 1931 XYZ color space.
5. A method of performing a fluorescence observation, wherein the method comprises:
filtering of an illumination light beam directed to an object using an illumination light filter, and
filtering of light emanating from the object using an observation light filter;
wherein a transmission characteristic of the observation light filter has, at wavelengths below a threshold wavelength, at least one wavelength range in which a transmission has values which are greater than a first value, and the transmission characteristic of the illumination light filter has, at wavelengths above the threshold wavelength, at least one wavelength range in which the transmission has values which are less than a fourth value;
wherein a transmission characteristic of the observation light filter has, at wavelengths above the threshold wavelength, at least one wavelength range in which the transmission has values greater than the first value, and the transmission characteristic of the observation light filter has, below the threshold wavelength, at least one wavelength range in which the transmission has values which are smaller than the fourth value; and
wherein:
∫
S
T
L
(
r
→
)
·
T
O
(
r
→
)
·
r
→
·
ⅆ
r
∫
S
T
L
(
r
→
)
·
T
O
(
r
→
)
·
ⅆ
r
=
R
→
and
W
→
-
R
→
≤
0.15
;
wherein:
λ designates the wavelength,
T L (λ) is the transmission characteristic of the illumination light filter,
T O (λ) is the transmission characteristic of the observation light filter, and
{right arrow over (r)} is a coordinate in the CIE xy chromaticity diagram of the CIE 1931 XYZ color space,
S is a line called the spectral locus in the CIE xy chromaticity diagram of the CIE 1931 XYZ color space, and
{right arrow over (W)} is the white point in the CIE xy chromaticity diagram of the CIE 1931 XYZ color space.
6. The set of filters according to claim 1 , wherein:
|{right arrow over (W)}−{right arrow over (R)}|≦0.10.
7. The fluorescence observation system according to claim 4 , wherein:
|{right arrow over (W)}−{right arrow over (R)}≦0.10.
8. The method according to claim 5 , wherein:
|{right arrow over (W)}−{right arrow over (R)}≦0.10.