Multiplex screening for lysosomal storage disorders (LSDS)
A novel protein profiling method of testing for Lysosomal Storage Diseases (“LSD”) using discovered normalized lysosomal fingerprint patterns. The fingerprint patterns reveal the health of lysosomal organelles, specific LSD, and clinical severity. Multiplexing bead technology for simultaneous screening of multiple LSD and normalizing measured enzyme activity or protein levels against other lysosomal proteins, enzymes, or enzyme activities. Compounds, reagents, and methods for identifying and quantifying multiple target enzymes and proteins.
1. A composition used for diagnosing a lysosomal storage disorder (“LSD”) comprising:
a set of capture antibodies conjugated to microspheres, each microsphere having a different specific capture antibody and having at least a first fluorophore and a second fluorophore wherein the first fluorophore and the second fluorophore are spectrally distinct, wherein the set of capture antibodies comprises antibodies capable of binding to α-iduronidase, α-glucosidase, LAMP-1, and saposin C; and
wherein each microsphere conjugated to a specific capture antibody has a specific ratio of the first fluorophore to the second fluorophore; and
wherein each microsphere conjugated to a specific capture antibody is spectrally distinct from a microsphere conjugated to a different capture antibody.
2. The composition of claim 1 , further comprising a detection antibody, wherein the detection antibody is capable of binding the target antigen, but is different from the capture antibody; and the detection antibody is conjugated to a detection label.
3. The composition of claim 1 , wherein the microsphere has a diameter of about 5 μm.
4. The composition of claim 1 , wherein the LSD is selected from the group consisting of Fabry; Mucopolysaccharidosis type I (“MPS I”); Mucopolysaccharidosis type II (“MPS-II”); Mucopolysaccharidosis type III (“MPS-III’); Mucopolysaccharidosis type IV (“MPS-IV”); and Glycogen storage disease II (“Pompe”).
5. The composition of claim 1 , wherein the LSD is selected from the group consisting of Gaucher disease types I/II/III; Cystinosis; Mucopolysaccharidosis type VI; Mucopolysaccharidosis type IVA; Niemann-Pick disease types A/B; Metachromatic leucodystrophy; Metachromatic leucodystrophy; Mucopolysaccharidosis type IIIA; Mucopolysaccharidosis type IIIB; Mucopolysaccharidosis type IIIC; Mucopolysaccharidosis type IIID; Mucopolysaccharidosis type VII; Mucopolysaccharidosis type IVB; Niemann-Pick disease type C1; Niemann-Pick disease type C2; Cholesterol ester storage disease; Aspartylglucosaminuria; GM1-Gangliosidosis types I/II/III; GM2-Gangliosidosis type I; GM2-Gangliosidosis type II; GM2-Gangliosidosis; Farber Lipogranulomatosis; β-Mannosidosis; Galactosialidosis types I/II; α-Mannosidosis types I/II; β-Mannosidosis; Mucolipidosis type I; Mucolipidosis types II/III; Mucolipidosis type IIIC; Mucolipidosis type IV; Multiple sulphatase deficiency; Neuronal Ceroid Lipofuscinosis, CLN1; Neuronal Ceroid Lipofuscinosis, CLN2; Neuronal Ceroid Lipofuscinosis, CLN3; Neuronal Ceroid Lipofuscinosis, CLN5; Neuronal Ceroid Lipofuscinosis, CLN8; Pycnodysostosis; and Sialic acid storage disease.