IP Library Granted Patent US 8,747,930
Granted Patent B2
US 8,747,930 · App. 12/494,170 · Granted Jun 10, 2014

Siliceous particles

Inventors: Daniel Fleischer (Oakland, CA); Andrew Thompson (Oakland, CA); Marko Jukic (San Francisco, CA); Guido Radaelli (Oakland, CA)
Assignee: Aurora Algae, Inc.
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Quick Facts
Patent No.
US 8,747,930
App. No.
12/494,170
Granted
Jun 10, 2014
Kind
B2
Abstract

Various aspects provide for extracting siliceous particles. Siliceous particles may include or be derived from diatoms. Certain embodiments provide for segregating suspensions into two or more segregation products. In some cases, a first product includes siliceous particles, and a second product may include hydrophobic species. Certain aspects provide for extracting non-siliceous biomass (e.g., lipids).

Claims (51)

1. A method for extracting siliceous particles, the method comprising:

providing a suspension comprising an aqueous liquid and diatoms having siliceous frustules;

adding a nonpolar solvent to the suspension to create a mixture;

agitating the mixture;

segregating the mixture into at least a first product and a second product, the first product having a higher amount of frustules than the second product; and

removing any of the first and second products, wherein the second product includes lipid biomass suitable for production of biofuel, wherein a ratio of the nonpolar solvent to the diatoms is below 1 percent by mass.

2. The method of claim 1 , wherein the provided suspension includes a concentration of diatoms in the liquid below 20 weight percent.

3. The method of claim 1 , wherein the provided suspension includes a concentration of diatoms in the liquid below 2 weight percent.

4. The method of claim 1 , wherein the liquid includes one or more dissolved salts.

5. The method of claim 1 , wherein the liquid includes seawater.

6. The method of claim 1 , wherein any of the diatoms includes members of the genera Navicula, Amphora, Thalassiosira, Chaetoceros, Nitzschia, Cyclotella, Skeletonema, Phaeodactylum, Achnanthes, Coscinodiscus, Cylindrotheca, Pseudo - Nitzschia, Thalassionema, Hantzschia, Cymbella, Psammodictyon , or any member of the Phylum Bacillariophyta.

7. The method of claim 1 , wherein any of the diatoms includes members of the genera Navicula, Amphora, Thalassiosira, Chaetoceros, Nitzschia, Cyclotella, Skeletonema or Phaeodactylum.

8. The method of claim 1 , wherein the nonpolar solvent includes an alkane.

9. The method of claim 8 , wherein the alkane includes hexane.

10. The method of claim 1 , wherein agitating includes ultrasonicating.

11. The method of claim 10 , wherein ultrasonicating includes ultrasonicating at an energy density between 10 and 1000 watts per liter of suspension.

12. The method of claim 1 , wherein segregating includes accelerating the mixture.

13. A nutrient substance made from siliceous particles fabricated according to a method comprising:

providing a suspension comprising an aqueous liquid and diatoms having siliceous frustules;

adding a nonpolar solvent to the suspension to create a mixture;

agitating the mixture;

segregating the mixture into at least a first product and a second product, the first product having a higher amount of frustules than the second product; and

removing the first product and wherein the second product includes lipid biomass suitable for production of biofuel, wherein a ratio of the nonpolar solvent to the diatoms is below 1 percent by mass.

14. The substance of claim 13 , wherein the method further comprises grinding the segregated frustules.

15. A method for providing bioavailable silica to growing organisms, the method comprising:

providing a suspension comprising an aqueous liquid and diatoms having siliceous frustules;

adding a nonpolar solvent to the suspension to create a mixture;

agitating the mixture;

segregating the mixture into at least a first product and a second product, the first product having a higher amount of frustules than the second product;

removing the first product; and

providing the frustules derived from the first product to the growing organisms and wherein the second product includes lipid biomass suitable for production of biofuel, wherein a ratio of the nonpolar solvent to the diatoms is below 1 percent by mass.

16. The method of claim 15 , wherein providing includes dissolving the frustules in a liquid in which the growing organisms are growing.

17. A method for extracting siliceous particles, the method comprising:

providing a suspension comprising an aqueous liquid and diatoms having siliceous frustules;

adding a nonpolar solvent to the suspension to create a mixture;

agitating the mixture;

segregating the mixture into at least a first product and a second product, the first product having a higher amount of frustules than the second product; and

removing any of the first and second products, wherein the second product includes lipid biomass suitable for production of biofuel, wherein a ratio of the nonpolar solvent to the diatoms is below 0.1 percent by mass.

18. A nutrient substance made from siliceous particles fabricated according to a method comprising:

providing a suspension comprising an aqueous liquid and diatoms having siliceous frustules;

adding a nonpolar solvent to the suspension to create a mixture;

agitating the mixture;

segregating the mixture into at least a first product and a second product, the first product having a higher amount of frustules than the second product; and

removing the first product and wherein the second product includes lipid biomass suitable for production of biofuel, wherein a ratio of the nonpolar solvent to the diatoms is below 0.1 percent by mass.

19. A method for providing bioavailable silica to growing organisms, the method comprising:

providing a suspension comprising an aqueous liquid and diatoms having siliceous frustules;

adding a nonpolar solvent to the suspension to create a mixture;

agitating the mixture;

segregating the mixture into at least a first product and a second product, the first product having a higher amount of frustules than the second product;

removing the first product; and

providing the frustules derived from the first product to the growing organisms and wherein the second product includes lipid biomass suitable for production of biofuel, wherein a ratio of the nonpolar solvent to the diatoms is below 0.1 percent by mass.

Assignments (4)
RELEASE OF SECURITY INTEREST Recorded Apr 20, 2015
From: SILICON VALLEY BANK, AS AGENT
To: AURORA ALGAE, INC.
Reel/Frame 035452/0305 →
SECURITY INTEREST Recorded Nov 8, 2011
From: AURORA ALGAE, INC.
To: SILICON VALLEY BANK
Reel/Frame 027249/0001 →
CHANGE OF NAME Recorded Aug 26, 2011
From: AURORA BIOFUELS, INC.
To: AURORA ALGAE, INC.
Reel/Frame 026817/0720 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 2, 2009
From: FLEISCHER, DANIEL; THOMPSON, ANDREW; JUKIC, MARKO; RADAELLI, GUIDO
To: AURORA BIOFUELS
Reel/Frame 023322/0485 →
Continuity (1)
Related Publication 20100330658A1 · Dec 30, 2010