Cartridge for biochemical analyses, system for biochemical analyses, and method of carrying out a biochemical process
A cartridge for biochemical analysis includes a support, a structure, which is set on the support and contains wells for receiving a solution, and photodetectors on the support, in positions corresponding to respective wells.
1. A system for biochemical analyses comprising:
an analyzer comprising a light source configured to illuminate a plurality of positions with light radiation, wherein the analyzer is configured to receive a plurality of wells such that each position is occupied by a respective well and is configured to receive a plurality of photodetectors such that each position is occupied by a respective photodetector, the analyzer further comprising an image sensor configured to acquire images of the plurality of wells occupying the plurality of positions and illuminated with the light radiation from the light source; and
a control unit configured to use the photodetectors to determine for each position an incident optical power of the light radiation from the light source based on an output of a photodetector occupying that position, to obtain the images of the illuminated wells from the image sensor, and to compensate the images of the illuminated wells as a function of the incident optical power determined for the positions.
2. The system according to claim 1 , wherein the plurality of wells and the plurality of photodetectors are located in a cartridge such that each well has a corresponding photodetector, and wherein the analyzer is configured to receive the cartridge such that each position is occupied by a respective well and a respective photodetector.
3. The system according to claim 2 , wherein the control unit is configured to determine a temperature at the wells through the photodetectors.
4. The system according to claim 1 , wherein the plurality of wells is located in a microreactor cartridge and the plurality of photodetectors is located in a calibration cartridge separate from the microreactor cartridge, and wherein the analyzer is configured to receive the microreactor cartridge such that each position is occupied by a respective well and is configured to receive the calibration cartridge such that each position is occupied by a respective photodetector.
5. The system according to claim 1 , further comprising a memory module accessible by the control unit, wherein the memory module contains for each photodetector:
a first response curve, calibrated and normalized as a function of wavelength;
a second response curve, calibrated and normalized as a function of angle of incidence; and
an incidence angle of the light radiation coming from the light source.
6. The system according to claim 5 , wherein the control unit is configured to determine the incident optical power for each position using the respective first and second response curves for the photodetector occupying that position.
7. The system according to claim 1 , wherein the control unit is housed in the analyzer.
8. A system for biochemical analyses comprising:
a cartridge, wherein the cartridge comprises a plurality of wells and a plurality of photodetectors such that each well has a corresponding photodetector;
an analyzer configured to receive the cartridge, wherein the analyzer comprises a light source configured to illuminate the plurality of wells with light radiation, wherein the analyzer further comprises an image sensor configured to acquire images of the plurality of wells illuminated by the light radiation from the light source; and
a control unit configured to use the photodetectors to determine for each well an incident optical power of the light radiation from the light source based on an output of the photodetector corresponding to that well, to obtain the images of the illuminated wells from the image sensor, and to compensate the images of the illuminated wells as a function of the incident optical power determined for the wells.
9. The system according to claim 8 , further comprising a memory module accessible by the control unit, wherein the memory module contains for each photodetector:
a first response curve, calibrated and normalized as a function of wavelength;
a second response curve, calibrated and normalized as a function of angle of incidence; and
an incidence angle of the light radiation coming from the light source.
10. The system according to claim 9 , wherein the control unit is configured to determine the incident optical power for each well using the respective first and second response curves for the photodetector corresponding to that well.
11. The system according to claim 8 , wherein the control unit is housed in the analyzer.
12. A system for biochemical analyses comprising:
an analyzer comprising a light source configured to illuminate a plurality of positions with light radiation, wherein the analyzer is configured to receive a plurality of wells such that each position is occupied by a respective well and is configured to receive a plurality of photodetectors such that each position is occupied by a respective photodetector, the analyzer further comprising an image sensor configured to acquire images of the plurality of wells occupying the plurality of positions and illuminated with the light radiation from the light source;
a control unit configured to use the photodetectors to determine for each position an incident optical power of the light radiation from the light source based on an output of a photodetector occupying that position, to obtain the images of the illuminated wells from the image sensor, and to compensate the images of the illuminated wells as a function of the incident optical power determined for the positions; and
a memory module accessible by the control unit, wherein the memory module contains for each photodetector:
a first response curve, calibrated and normalized as a function of wavelength;
a second response curve, calibrated and normalized as a function of angle of incidence;
an incidence angle of the light radiation coming from the light source; and
an incident optical power of the light radiation from the light source determined by the control unit.
13. The system according to claim 12 , wherein the plurality of wells and the plurality of photodetectors are located in a cartridge such that each well has a corresponding photodetector, and wherein the analyzer is configured to receive the cartridge such that each position is occupied by a respective well and a respective photodetector.
14. The system according to claim 12 , wherein the plurality of wells is located in a microreactor cartridge and the plurality of photodetectors is located in a calibration cartridge separate from the microreactor cartridge, and wherein the analyzer is configured to receive the microreactor cartridge such that each position is occupied by a respective well and is configured to receive the calibration cartridge such that each position is occupied by a respective photodetector.