Modified phage for displaying post-translationally modified proteins and uses thereof
Disclosed herein are modified phage comprising a fusion protein located on the surface of the phage wherein the fusion protein comprises a surface protein and a post-translationally modified protein. Also disclosed are methods of making and using modified phage comprising post-translationally modified proteins located on the surface of the phage.
1. A method for displaying a post-translationally modified polypeptide on the surface of a phage comprising
(a) providing a mammalian host cell comprising one or more expression vectors encoding a plurality of fusion polypeptides, wherein each expression vector comprises a nucleic acid encoding a fusion polypeptide and wherein the fusion polypeptide comprises a surface polypeptide and a heterologous polypeptide capable of being post-translationally modified;
(b) culturing the mammalian host cell under conditions that allow the fusion polypeptides to be expressed and that allow the heterologous polypeptides to be post-translationally modified;
(c) isolating the fusion polypeptides; and
d) incubating the phage with the fusion polypeptides wherein the cell is transformed with one or more expression vectors encoding a plurality of fusion polypeptides and wherein the cell expresses a first fusion polypeptide comprising SF162 Env and gpV or gpD and a second fusion polypeptide comprising YU2 Env and gpV or gpD.