Method for cloning pluripotent stem cells
View Patent ↗Embodied herein are methods of reprogramming somatic cells or tissue stem cells to a more multipotent state or even a pluripotent state, the methods do not involve gene transfer of master transcription factor genes/proteins. The methods are also useful for rapid and efficient cloning of induced pluripotent stem cells after gene transfer of master transcription factor genes/proteins.
1. A method of reprogramming a population of tissue stem cells comprising the steps of:
a. contacting a population of cells comprising tissue stem cells with xanthine (Xn) in a culture medium, wherein the tissue stem cells respond to Xn by undergoing symmetric cell division;
b. culturing the population of cells of step a in the culture medium containing Xn for a sufficient amount of time to expand the number of tissue stem cells by suppression of asymmetric cell kinetics in the presence of Xn; and
c. culturing the population of cells comprising tissue stem cells from step b in a medium capable of supporting the maintenance of pluripotent cells, thereby reprogramming the cells of step (b) to a pluripotent state.
2. The method of claim 1 , wherein Xn is included in the medium capable of supporting the maintenance of pluripotent cells.
3. A method of reprogramming a tissue stem cell comprising the steps of:
a. clonally isolating a tissue stem cell from a population of cells by culturing the population of cells in a proliferation culture medium comprising xanthine (Xn) to suppress asymmetric kinetics and isolating a tissue stem cell that responds to Xn by undergoing symmetric cell division;
b. culturing the isolated cell of step a in a proliferation culture medium comprising xanthine for a sufficient time to expand the number of cells by suppression of asymmetric cell kinetics in the presence of Xn; and
c. culturing the cells of step b) in a medium capable of supporting the maintenance of pluripotent cells thereby reprogramming the tissue stem cell to a pluripotent state.
4. The method of claims 3 , wherein the medium capable of supporting the maintenance of pluripotent cells comprises Xn.
5. The method of claim 1 , wherein the concentration of xanthine is about 1 mM.
6. The method of claim 1 , wherein the tissue stem cell is selected from the group of liver stem cells, hair follicle stem cells and pancreas stem cells.
7. A method of reprogramming a tissue stem cell comprising contacting a tissue stem cell with xanthine in a medium capable of supporting the maintenance of pluripotent cells, wherein the tissue stem cell responds to Xn by undergoing symmetric cell division.
8. The method of claim 7 , wherein the concentration of xanthine is about 1 mM.
9. The method of claim 7 , wherein the tissue stem cell is selected from the group of liver stem cells, hair follicle stem cells and pancreas stem cells.