Compositions and methods using herpes simplex virus
View Patent ↗Provided herein are methods and compositions for use in treating HSV-related conditions and diseases.
1. An attenuated HSV-1 virus comprising a deletion that removes a fragment of only the amino-terminal domain of the γ 1 34.5 protein, wherein the γ 1 34.5 protein comprising the deletion is capable of being expressed by the virus, and wherein the γ 1 34.5 protein does not interact with TBK1.
2. The attenuated HSV-1 virus of claim 1 , wherein the deletion is present in at least one copy of a γ 1 34.5 polynucleotide.
3. The attenuated HSV-1 virus of claim 1 , wherein the deletion comprises a fragment selected from the group consisting of amino acid 1 to amino acid 146 of SEQ ID NO: 3 and amino acid 72 to amino acid 106 of SEQ ID NO: 3.
4. An immunogenic composition comprising the virus of claim 1 and an adjuvant.
5. The composition of claim 4 , wherein the adjuvant is selected from the group consisting of a sterile oil-in-water emulsion free of animal origin ingredients, wherein the emulsion comprises uniformly dispersed, micron size oil droplets and dimethyldioctadecylammonium bromide; monophosphoryl lipid A (MPL); and Freund.
6. A method of provoking an immune response against a HSV-1 strain in a subject comprising the step of administering to the subject the immunogenic composition of claim 4 .
7. The method of claim 6 , wherein the subject is human.
8. The attenuated HSV-1 virus of claim 3 , wherein the deletion consists of amino acid 72 to amino acid 106 of SEQ ID NO: 3.
9. The attenuated HSV-1 virus of claim 3 , wherein the deletion consists of amino acid 1 to amino acid 146 of SEQ ID NO: 3.