Enzymatic synthesis of sulfated polysaccharides
Heparin is synthesized from a polysaccharide comprised of a 1-4 glycosidically linked alternating polymer of uronic acid and glucosamine residues, wherein the uronic acid is selected from iduronic and glucuronic acid, wherein the glucosamine is partially N-sulfated; by a series of selective reactions catalyzed by recombinant enzymes.
1. A method of preparing heparin from a first polysaccharide comprised of 1-4 glycosidically linked alternating polymer of uronic acid and glucosamine residues, wherein the uronic acid is selected from iduronic and glucuronic acid, wherein the glucosamine is partially N-sulfated; the method comprising
(a) treating the first polysaccharide with a reaction mixture comprising recombinant 2-O-sulfotransferase (OST) enzyme comprising the amino acid sequence of SEQ ID NO: 1, to yield a second polysaccharide;
(b) treating the second polysaccharide with a reaction mixture comprising recombinant 6-O-sulfotransferase (OST) enzyme to yield a third polysaccharide;
(c) treating the third polysaccharide with a reaction mixture comprising recombinant 3-O-sulfotransferase (OST) enzyme isoform 1 to yield heparin;
wherein in each of steps (a)-(c) the reaction mixture further comprises 3′-phosphoadenosine 5′-phosphosulfate (PAPS), an arylsulfotransferase and an aryl sulfate.
2. The method of claim 1 , wherein the arylsulfotransferase is arylsulfotransferase IV and the aryl sulfate is p-nitrophenol sulfate (PNPS).
3. The method of claim 1 , wherein said incubating is for from about 1 minute to about 30 minutes.
4. The method of claim 1 , wherein the recombinant 6-O-sulfotransferase (OST) comprises the amino acid sequence selected from SEQ ID NOs 5, 6 or 7.
5. The method of claim 1 , wherein the recombinant 3-O-sulfotransferase (OST) isoform 1 comprises the amino acid sequence of SEQ ID NO: 2.
6. The method of claim 5 , wherein the recombinant 6-O-sulfotransferase (OST) comprises the amino acid sequence selected from SEQ ID NOs 5, 6 or 7.