Fluorogenic hydrazine-substituted compounds

1. A kit for detecting an analyte in a sample, wherein the analyte contains an aldehyde group or a ketone group, wherein the kit comprises:

a compound of Formula I that binds the analyte:

or a stereoisomer, tautomer, hydrate, solvate, or salt thereof;

wherein,

X is H;

R 1 is selected from the group consisting of H, alkyl and substituted alkyl;

R 2 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 and R 10 are each independently selected from the group consisting of H, alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, acylamino, acyloxy, amino, substituted amino, aminocarbonyl, aminothiocarbonyl, aminocarbonylamino, aminothiocarbonylamino, aminocarbonyloxy, aminosulfonyl, aminosulfonyloxy, aminosulfonylamino, amidino, carboxyl, carboxyl ester, (carboxyl ester)amino, (carboxyl ester)oxy, cyano, halo, hydroxy, nitro, SO 3 − , sulfonyl, substituted sulfonyl, sulfonyloxy, thioacyl, thiol, alkylthio, substituted alkylthio, aryl, substituted aryl, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloalkyl, heterocyclyl, and substituted heterocyclyl;

R 3 is selected from the group consisting of alkoxy, substituted alkoxy, amino, substituted amino, hydrazinyl, alkyl, substituted alkyl, acyl, acylamino, acyloxy, aminocarbonyl, aminothiocarbonyl, aminocarbonylamino, aminothiocarbonylamino, aminocarbonyloxy, aminosulfonyl, aminosulfonyloxy, aminosulfonylamino, amidino, carboxyl, carboxyl ester, (carboxyl ester)amino, (carboxyl ester)oxy, cyano, halo, hydroxy, nitro, SO 3 − , sulfonyl, substituted sulfonyl, sulfonyloxy, thioacyl, thiol, alkylthio, substituted alkylthio, aryl, substituted aryl, heteroaryl, substituted heteroaryl, cycloalkyl, substituted cycloalkyl, heterocyclyl, and substituted heterocyclyl; or

R 2 and R 3 , R 3 and R 4 , or R 2 , R 3 and R 4 are taken together to form a fused heterocyclyl group, a fused substituted heterocyclyl group, a fused aryl group, a fused substituted aryl group, a fused heteroaryl group or a fused substituted heteroaryl group; and

instructions for detecting the analyte comprising the steps of:

contacting the sample with the compound;

incubating the sample and the compound for a sufficient amount of time to form a complex between the compound and analyte;

illuminating the complex with an appropriate wavelength to form an illuminated complex; and

detecting emissions from the illuminated complex;

wherein the hydrate or solvate thereof is a hydrate or solvate of an aqueous solution, an aqueous miscible solution, or a water-miscible organic solvent.

2. The kit of claim 1 , further comprising instructions for covalently bonding the compound to the analyte.

3. The kit of claim 1 , further comprising one or more of the following: a buffering agent, a purification medium, a vial comprising the analyte, or an organic solvent.

4. The kit of claim 1 , wherein the analyte is malondialdehyde or 4-hydroxynonenal.

5. The kit of claim 1 , wherein the analyte comprises an aldehyde group.

6. The kit of claim 1 , wherein the sample comprises live cells, intracellular fluids, extracellular fluids, biological fluids, biological fermentation media, environmental sample, industrial samples, proteins, peptides, buffer solutions, or biological fluids.

7. The kit of claim 1 , wherein the sample comprises blood cells, immune cells, cultured cells, muscle tissue, neurons, extracellular vesicles, vascular tissue, blood fluids, saliva, urine, water, soil, waste water, sea water, pharmaceuticals, foodstuffs or beverages.

8. The kit of claim 1 , wherein the sample is immobilized on a polymeric membrane, within a polymeric gel, on a microparticle, on a microarray, on a silicon chip, on a glass slide, on a microwell plate, or on a microfluidic chip.