Genomically multiplied rapeseed plants, compositions derived therefrom and uses of same
A rapeseed plant having a multiplied genome being at least as fertile as a euploid rapeseed plant isogenic to the genomically multiplied plant grown under similar conditions.
1. A Brassica napus rapeseed plant having a doubled amphidiploid genome being at least as fertile as a euploid Brassica napus rapeseed plant isogenic to the genomically doubled plant grown under similar conditions, wherein fertility is determined by number of seeds per plant, and wherein the genomically doubled plant is grown from genomically doubled rapeseed seed, which seed have been generated by:
(a) applying a magnetic field to seeds of the euploid Brassica napus ; and
(b) contacting said seeds of the euploid Brassica napus with a microtubule polymerization inhibitor under said magnetic field for targeted delivery of said microtubule polymerization inhibitor to chromatin fibers in nuclei of said seeds.
2. The plant of claim 1 , exhibiting genomic stability for at least 2 generations.
3. The plant of claim 1 , having a seed weight per 40 seeds exceeding that of said euploid rapeseed plant.
4. A rapeseed plant as deposited under the Budapest treaty in NCIMB Ltd. and having Accession No. NCIMB 41592 Brassica napus 187-24N.
5. A plant part of the rapeseed plant of claim 1 .
6. Rapeseed meal produced from the plant of claim 1 .
7. THE plant part of claim 5 being a seed.
8. An isolated regenerable cell of the rapeseed plant of claim 1 .
9. The cell of claim 8 , exhibiting genomic stability for at least 2 generations in culture.
10. The cell of claim 8 being from a meristem, pollen, a leaf, a root, a root tip, an anther, a pistil, a flower, a seed or a stem.
11. A tissue culture comprising the regenerable cell of claim 8 .
12. A method of producing seeds of rapeseed, comprising self-breeding or cross-breeding the plant of claim 1 .
13. A method of producing rapeseed oil, the method comprising:
(a) harvesting seeds of the rapeseed plant of claim 1 ; and
(b) processing said seeds so as to produce the rapeseed oil.
14. A method of generating a genomically doubled Brassica napus rapeseed seed, the method comprising:
(a) applying a magnetic field to seeds of a euploid Brassica napus ; and
(b) contacting said seeds of said euploid Brassica napus with a G2/M cell cycle inhibitor comprising a microtubule polymerization inhibitor under said magnetic field for targeted delivery of said microtubule polymerization inhibitor to chromatin fibers in nuclei of said seeds;
thereby generating the genomically doubled Brassica napus rapeseed seed.
15. The method of claim 14 , wherein said microtubule polymerization inhibitor is selected from the group consisting of colchicine, nocodazole, oryzaline, trifluraline and vinblastine sulphate.
16. A tissue culture comprising the regenerable cell of claim 10 .
17. The plant of claim 1 , wherein said microtubule polymerization inhibitor is selected from the group consisting of colchicine, nocodazole, oryzaline, trifluraline and vinblastine sulphate.
18. The plant part of claim 7 , wherein said seed is a hybrid seed.