Rapid preparation of stem cell matrices for use in tissue and organ treatment and repair
A rapid method for preparing stem cell and physiologically acceptable matrix compositions for use in tissue and organ repair is described. Compared with previous tissue engineering materials, the stem cell-matrix compositions of the present invention do not require long-term incubation or cultivation in vitro prior to use in in vivo applications. The stem cells can be from numerous sources and may be homogeneous, heterogeneous, autologous, and/or allogeneic in the matrix material. The stem cell-matrix compositions provide point of service utility for the practitioner, wherein the stem cells and matrix can be combined not long before use, thereby alleviating costly and lengthy manufacturing procedures. In addition, the stem cells offer unique structural properties to the matrix composition which improves outcome and healing after use. Use of stem cells obtained from muscle affords contractility to the matrix composition.
1. A method of repairing or healing stress urinary incontinence comprising:
a) admixing a preparation comprising muscle-derived progenitor cells (MDCs) with a small intestine submucosa (SIS) to form a stem cell matrix dressing; and
b) incubating the stem cell matrix dressing in vitro for less than about 12 hours prior to placing as a suburethral sling at a urinary tract sphincter; and
c) supporting the sphincter with the stem cell matrix, for treating contractility of the sphincter, thereby repairing or healing said stress urinary incontinence.
2. The method according to claim 1 , wherein the muscle-derived progenitor cells are autologous to a recipient.
3. The method according to claim 1 , wherein the muscle-derived progenitor cells are allogeneic to a recipient.
4. The method according to claim 1 , wherein the stem cell matrix dressing is contractible.
5. The method according to claim 1 , wherein about 2.5×10 3 to about 1×10 6 muscle-derived progenitor cells are admixed with the small intestine submucosa (SIS).
6. The method according to claim 1 , wherein about 5×10 3 to about 1×10 6 muscle-derived progenitor cells are admixed with the small intestine submucosa (SIS).
7. The method according to claim 1 , wherein about 1×10 5 muscle-derived progenitor cells are admixed per 1 cm 2 of the small intestine submucosa (SIS).
8. The method according to claim 1 , wherein the stem cell matrix dressing is incubated in vitro for less than about 3 hours prior to placing as the suburethral sling.
9. The method according to claim 1 , wherein the stem cell matrix dressing is incubated in vitro for less than about 1 hour prior to placing as the suburethral sling.
10. The method according to claim 1 , wherein the stem cell matrix dressing is incubated in vitro for less than about 30 minutes prior to placing as the suburethral sling.
11. The method according to claim 1 , wherein the stem cell matrix dressing is incubated in vitro from about 5 seconds to about 30 minutes prior to placing as the suburethral sling.