Methods and devices for separating liquid components
Methods and devices for preparing a solid-fibrin web are provided. One method may include drawing blood from a patient, separating plasma from the blood, contacting the plasma with a calcium-coagulation activator and concurrently coagulating and axially centrifuging the plasma to form the solid-fibrin web. The solid-fibrin web may be suitable for regenerating body tissue in a living organism. Devices used in the methods may also be provided.
1. A device for use in axial centrifugation, the device comprising:
a container configured for axial centrifugation, the container including
a primary chamber configured to receive blood through a port, the primary chamber including an anticoagulant;
a secondary chamber containing a coagulator; and
a filter separating the primary chamber from the secondary chamber, the filter substantially preventing red and white blood cells, originating from the blood drawn into the primary chamber, from entering the secondary chamber under a centrifugal force of about 1000×G or greater, but substantially permitting plasma and platelets originating from the blood to flow into the secondary chamber under a centrifugal force of about 1000×G or greater; and
wherein the secondary chamber optionally comprises a therapeutic enhancing agent.
2. The device of claim 1 , wherein the coagulator comprises zinc.
3. The device of claim 1 , wherein the coagulator comprises calcium.
4. The device of claim 3 , wherein the coagulator comprises at least one of calcium chloride, calcium fluoride, calcium carbonate, calcium gluconate, calcium fumarate, calcium pyruvate, an organic calcium salt and combinations thereof.
5. The container of claim 1 , wherein the therapeutic enhancing agent comprises at least one of an antibiotic, analgesic, cancer therapeutic, platelet-growth factor, bone morphogenic protein, stem cell, bone graft material, soft tissue graft, platelet-derived growth factor cell culture material, immunosuppressant and a combination thereof.
6. A container for use in axial centrifugation, the container comprising:
a primary chamber that can receive blood, the primary chamber including a separation medium comprising at least one of a silicone gel, polyester gel, thixotropic gel and a combination thereof;
a secondary chamber containing a coagulator; and
a diaphragm positioned between the primary chamber and the secondary chamber, the diaphragm including at least one vent initially plugged with the separation medium to prevent fluid communication between the primary chamber and the secondary chamber, the vent configured to open when the separation medium moves into the primary chamber to separate red and white blood cells from platelet rich plasma during centrifugation of at least about 1000×G to thereby allow the platelet rich plasma to flow into the secondary chamber after centrifugation; wherein
the secondary chamber comprises a removable bottom, and
wherein the primary chamber is above the secondary chamber during centrifugation.
7. The container of claim 6 , wherein the primary chamber has a first circumference and the secondary chamber has a second circumference, the first circumference and the second circumference being substantially the same.
8. The container of claim 6 , wherein at least one of the primary chamber and secondary chamber contains a therapeutic enhancing agent.
9. The container of claim 8 , wherein the therapeutic enhancing agent comprises at least one of an antibiotic, analgesic, cancer therapeutic, platelet-growth factor, bone morphogenic protein, stem cell, bone graft material, soft tissue graft, platelet-derived growth factor cell culture material, immunosuppressant and a combination thereof.
10. A device for use in axial centrifugation, the device comprising:
a container configured for axial centrifugation, the container capable of holding a fluid, the container including
a first chamber that can receive blood, the first chamber including a separation medium comprising at least one of a silicone gel, polyester gel, thixotropic gel and a combination thereof, the first chamber having a first circumference;
a second chamber having a second circumference and containing a coagulator, the second circumference being greater than the first circumference;
a diaphragm positioned between the first chamber and the second chamber, the diaphragm including at least one vent initially plugged with the separation medium to prevent fluid communication between the primary chamber and the second chamber, the vent configured to open when the separation medium moves into the primary chamber to separate red and white blood cells from platelet rich plasma during centrifugation of at least about 1000×G to thereby allow the platelet rich plasma to flow into the second chamber after centrifugation; and wherein
the second chamber comprises a removable bottom.
11. The device of claim 10 , wherein the coagulator comprises calcium.
12. The device of claim 11 , wherein the activator comprises at least one of calcium chloride, calcium fluoride, calcium carbonate, calcium gluconate, calcium fumarate, calcium pyruvate, an organic calcium salt and combinations thereof.
13. The device of claim 10 , wherein the first chamber comprises an upper portion and a lower portion, and wherein the upper portion is separated from the lower portion by a separator substantially preventing fluid communication therebetween.
14. The device of claim 13 , wherein fluid communication is provided between the upper and lower portions when the device is centrifuged at about 1000×G or greater.
15. The device of claim 14 , wherein the lower portion of the first chamber is in fluid communication with the second chamber.
16. The device of claim 10 , further comprising a therapeutic agent.
17. The device of claim 10 , wherein the first chamber contains an anti-coagulant.