Diagnostic antibody assay
Diagnostic assays for the diagnosis of amyloidosis, in particular Alzheimer's disease, and related aspects. In particular, monoclonal antibodies and an antibody assay are provided.
1. An isolated monoclonal antibody that binds to AβpGlu(11) peptides or variants thereof, wherein
said antibody has a dissociation constant (K D ) value of 10 −7 M or less; and
the variable part of the light chain of said antibody is encoded by a nucleic acid molecule having the nucleotide sequence of SEQ ID NO: 51 or has the amino acid sequence of SEQ ID NO: 52.
2. The antibody according to claim 1 , wherein said antibody is Aβ 13-11-6 (Deposit No. DSM ACC 3100).
3. The antibody according to claim 1 , wherein the antibody is a labeled antibody.
4. The antibody according to claim 1 , wherein the antibody is immobilized on a solid phase.
5. An isolated monoclonal antibody that binds to AβpGlu(11) peptides or variants thereof, wherein:
said antibody has a dissociation constant (K D ) value of 10 −7 M or less, and
the variable part of the heavy chain of said antibody is encoded by a nucleic acid molecule having the nucleotide sequence of SEQ ID NO: 53 or has the amino acid sequence of SEQ ID NO: 54.
6. The antibody according to claim 5 , wherein said antibody is Aβ 13-11-6 (Deposit No. DSM ACC 3100).
7. An isolated monoclonal antibody that binds to AβpGlu(11) peptides or variants thereof, wherein:
said antibody has a dissociation constant (K D ) value of 10 −7 M or less,
the variable part of the light chain of said antibody is encoded by a nucleic acid molecule having the nucleotide sequence of SEQ ID NO: 51 or has the amino acid sequence of SEQ ID NO: 52, and
the variable part of the heavy chain of said antibody is encoded by a nucleic acid molecule having the nucleotide sequence of SEQ ID NO: 53 or has the amino acid sequence of SEQ ID NO: 54.
8. The antibody according to claim 7 , wherein said antibody is Aβ 13-11-6 (Deposit No. DSM ACC 3100).
9. An antibody obtainable from the hybridoma cell line DSM ACC 3100.
10. A hybridoma cell line DSM ACC 3100.