Method for the in vitro diagnosis or prognosis of testicular cancer
A method for in vitro diagnosis or prognosis of testicular cancer in a biological sample from a patient suspected of suffering from testicular cancer, having a step of detecting the presence or absence of methylation of CpG dinucleotides in at least one genomic DNA target sequence of the sample, the target sequence being selected from at least one of the sequences identified in SEQ ID NOS: 1 to 7 or from at least one sequence which exhibits at least 99% identity with one of the sequences identified in SEQ ID NOS: 1 to 7 and the sequences complementary thereto; to the DNA sequences and to the use thereof as a testicular cancer marker.
1. A method for in vitro diagnosis of testicular cancer, comprising:
obtaining a biological sample from a patient suspected of having testicular cancer;
performing an assay to determine the methylation status of CpG dinucleotides in a genomic DNA target sequence, the DNA target sequence being at least one sequence selected from the group consisting of sequences having at least 99% sequence identity with the full-length sequences of SEQ ID NOS: 1-5 and the sequences fully complementary thereto; and
diagnosing the patient with testicular cancer when the DNA target sequence is hypomethylated as compared to a methylation status indicative of the absence of testicular cancer,
wherein the assay comprises:
extracting genomic DNA from the biological sample;
treating the extracted genomic DNA to convert cytosine bases of CpG dinucleotides that are nonmethylated at position 5 into uracil bases;
amplifying the treated genomic DNA target sequence; and
determining the methylation status of the CpG dinucleotides in the genomic DNA target sequence from the amplified genomic DNA target sequence.
2. The method of claim 1 , wherein the extracted genomic DNA is treated using hydrogen sulfite, disulfite, bisulfite, or a combination thereof.
3. The method of claim 1 , wherein the treated genomic DNA target sequence is amplified using at least one primer comprising a sequence selected from the group consisting of the full-length sequences of SEQ ID NOS: 26-45.
4. The method of claim 1 , wherein the biological sample is a testicular tissue extract or a biological fluid.
5. The method of claim 1 , wherein the biological sample is blood, serum, plasma, urine, or seminal fluid.
6. The method of claim 1 , wherein the DNA target sequence is hypomethylated if 60% or less of the CpG dinucleotides are methylated.
7. The method of claim 1 , wherein the DNA target sequence is hypomethylated if 30% or less of the CpG dinucleotides are methylated.
8. A method for in vitro diagnosis of testicular cancer, comprising: obtaining a biological sample from a patient suspected of having testicular cancer; performing an assay to determine the methylation status of CpG dinucleotides in a genomic DNA target sequence, the DNA target sequence being at least one sequence selected from the group consisting of the 5′ LTR U3 promoter sequences of the HW4TT, HW2TT, HW 13TT, HWXTT, and HW21TT loci; and diagnosing the patient with testicular cancer when the DNA target sequence is hypomethylated as compared to a methylation status indicative of the absence of testicular cancer, wherein the assay comprises: extracting genomic DNA from the biological sample; treating the extracted genomic DNA to convert cytosine bases of CpG dinucleotides that are nonmethylated at position 5 into uracil bases; amplifying the treated genomic DNA target sequence; and determining the methylation status of the CpG dinucleotides in the genomic DNA target sequence from the amplified genomic DNA target sequence.
9. The method of claim 8 , wherein the extracted genomic DNA is treated using hydrogen sulfite, disulfite, bisulfite, or a combination thereof.
10. The method of claim 8 , wherein the treated genomic DNA target sequence is amplified using at least one primer comprising a sequence selected from the group consisting of the full-length sequences of SEQ ID NOS: 26-45.
11. The method of claim 8 , wherein the biological sample is a testicular tissue extract or a biological fluid.
12. The method of claim 8 , wherein the biological sample is blood, serum, plasma, urine, or seminal fluid.
13. The method of claim 8 , wherein the DNA target sequence is hypomethylated if 60% or less of the CpG dinucleotides are methylated.
14. The method of claim 8 , wherein the DNA target sequence is hypomethylated if 30% or less of the CpG dinucleotides are methylated.