A fibrin sealant, comprises (a) thrombin, (b) fibrinogen, (c) polyP, and (d) calcium. The thrombin and the fibrinogen are separated prior to application.
1. A fibrin sealant composition, comprising:
(a) a first container containing a first composition comprising isolated or recombinant thrombin, and
(b) a second container containing a second composition comprising: purified or recombinant fibrinogen, polyphosphate (polyPn), wherein n is at least 25, and calcium.
2. The fibrin sealant composition of claim 1 , wherein n is 25-1000.
3. The fibrin sealant composition of claim 1 , further comprising a cross-linking agent.
4. The fibrin sealant composition of claim 3 , wherein the cross-linking agent is factor XIII.
5. The fibrin sealant composition of claim 1 , further comprising a fibrinolysis inhibitor.
6. The fibrin sealant composition of claim 1 , wherein the fibrinogen is present in an amount between 2.5 to 200 mg/mL.
7. A fibrin sealant kit, comprising:
(I) a first container containing a first composition comprising isolated or recombinant thrombin,
(II) a second container containing a second composition, stored separate from the first composition, comprising: purified or recombinant fibrinogen, polyphosphate (polyPn), wherein n is at least 25, and calcium, and
(III) a fibrin sealant applicator.
8. The fibrin sealant kit of claim 7 , wherein n is 25-1000.
9. The fibrin sealant kit of claim 7 , further comprising a cross-linking agent.
10. The fibrin sealant kit of claim 9 , wherein the cross-linking agent is factor XIII.
11. The fibrin sealant kit of claim 7 , further comprising a fibrinolysis inhibitor.
12. The fibrin sealant kit of claim 7 , wherein the fibrin sealant applicator is a double barreled syringe.
13. The fibrin sealant kit of claim 7 , wherein the fibrin sealant applicator is a spray applicator.
14. The fibrin sealant composition of claim 1 , wherein the fibrinogen is recombinant fibrinogen.
15. The fibrin sealant composition of claim 1 , wherein the first composition and second composition are liquid.
16. The fibrin sealant composition of claim 1 , wherein the fibrinogen, polyPn and calcium have been incubated for at least 15 minutes.
17. The fibrin sealant kit of claim 7 , wherein the fibrinogen is recombinant fibrinogen.
18. The fibrin sealant kit of claim 7 , wherein the first composition and second composition are liquid.
19. The fibrin sealant kit of claim 7 , wherein the fibrinogen, polyPn and calcium have been incubated for at least 15 minutes.
20. The fibrin sealant kit of claim 7 , wherein the fibrinogen is present in an amount between 2.5 to 200 mg/mL.