In vitro method for diagnosing or predicting hypertension and/or early-stage cardiovascular end-organ damage by determining the concentration of soluble prorenin receptor
A method for diagnosis and/or prediction of hypertension and/or cardiovascular end-organ damage is disclosed. The method disclosed comprises the determination of the presence of soluble pro-renin receptor in samples derived from a subject. Furthermore, the use of anti-sPRR antibodies in diagnosis and/or prediction of cardiovascular end-organ damage is encompassed.
1. An in vitro method for diagnosis or prediction of hypertension and/or early-stage cardiovascular end-organ damage in a patient, the method comprising determining the concentration of soluble prorenin receptor (sPRR) in a sample from the patient using an antibody or antibody fragment that immunologically binds a polypeptide comprising SEQ ID NO:4 wherein the presence of the polypeptide at increased concentration compared to the concentration of such a polypeptide in samples from healthy subjects is indicative for hypertension and/or early-stage cardiovascular end-organ damage and wherein the presence of the polypeptide at decreased concentration compared to the concentration of such a polypeptide in samples from healthy subjects is indicative for late stage cardiovascular end-organ damage.
2. The in vitro method according to claim 1 , wherein the presence of said polypeptide at a concentration of at least 3 ng/ml is indicative of the hypertension and/or early-stage cardiovascular end-organ disease.
3. The method according to claim 2 , wherein the presence of said polypeptide at a concentration of at least 4 ng/ml is indicative of the hypertension and/or early-stage cardiovascular end-organ disease.
4. The method according to claim 2 , wherein the presence of said polypeptide at a concentration of at least 5 ng/ml is indicative of the hypertension and/or early-stage cardiovascular end-organ disease.
5. The method according to claim 2 , wherein the presence of said polypeptide at a concentration of at least 10 ng/ml is indicative of the hypertension and/or early-stage cardiovascular end-organ disease.
6. The in vitro method according to claim 1 , wherein the sample is selected from the group comprising a blood sample, a serum sample, and a plasma sample.
7. The in vitro method according to claim 1 , wherein the immunoassay is selected from the group of an immuno-precipitation assay, an enzyme immunoassay (EIA), a radioimmunoassay (RIA) or a fluorescent immunoassay, a chemiluminescent assay, an agglutination assay, a nephelometric assay, a turbidimetric assay, a Western blot, a competitive immunoassay, a noncompetitive immunoassay, a homogeneous immunoassay, a heterogeneous immunoassay, a bioassay and a reporter-assay such as a Luciferase-Assay.
8. The in vitro method according to claim 1 , wherein the immunoassay is an enzyme-linked immunosorbent assay (ELISA).
9. An in vitro method for determining the concentration of soluble prorenin receptor (sPRR) in a sample from a patient suspected of developing hypertension and/or early stage cardiovascular end-organ disease, the method comprising conducting an immunoassay that determines the level of a polypeptide comprising SEQ ID NO:2, SEQ ID NO:3 or SEQ ID NO:4 in such a sample.
10. The in vitro method according to claim 9 , wherein the concentration of the polypeptide is determined to be at least 3 ng/ml.
11. The in vitro method according to claim 10 , wherein the concentration of the polypeptide is determined to be at least 4 ng/ml.
12. The in vitro method according to claim 11 , wherein the concentration of the polypeptide is determined to be at least 5 ng/ml.
13. The in vitro method according to claim 9 , wherein the sample is selected from the group comprising a blood sample, a serum sample, and a plasma sample.
14. The in vitro method according to claim 9 , wherein the immunoassay is selected from the group of an immuno-precipitation assay, an enzyme immunoassay (EIA), a radioimmunoas say (RIA) or a fluorescent immunoassay, a chemiluminescent assay, an agglutination assay, a nephelometric assay, a turbidimetric assay, a Western blot, a competitive immunoassay, a noncompetitive immunoassay, a homogeneous immunoassay, a heterogeneous immunoassay, a bioassay and a reporter-assay such as a Luciferase-Assay.
15. The in vitro method according to claim 14 , wherein the immunoassay is an ELISA.
16. The in vitro method according to claim 9 , wherein the immunoassay determines the level of a polypeptide comprising SEQ ID NO:2 is such a sample.
17. The in vitro method according to claim 9 , wherein the immunoassay determines the level of a polypeptide comprising SEQ ID NO:3 is such a sample.
18. The in vitro method according to claim 9 , wherein the immunoassay determines the level of a polypeptide comprising SEQ ID NO:4 is such a sample.