Induced heterosis related mutations
The present invention provides plant inbred mutant parent lines producing hybrid offspring having at least one heterosis-related phenotype, particularly total yield associated phenotypes, methods for identifying same and hybrid plants produced therefrom. The present invention further discloses heterosis-related genes and provides the corresponding isolated polynucleotides.
1. A method for producing an angiosperm hybrid plant showing at least one yield-related heterotic phenotype, comprising:
a. providing a mutant parent inbred angiosperm plant comprising a single mutation in a SINGLE FLOWER TRUSS (SFT) gene, wherein the mutation is such that it reduces SFT gene function when homozygous;
b. providing a parent inbred angiosperm plant, homozygous for wild-type alleles of the SFT gene, the wild-type gene encoding a protein having the amino acid sequence set forth in SEQ ID NO:1; and
c. crossing the mutant parent inbred angiosperm plant with the parent inbred angiosperm plant, homozygous for wild-type alleles of said SFT gene;
thereby producing a heterozygous hybrid angiosperm plant comprising said wild-type SET allele and a mutated sft allele, wherein the heterozygous hybrid angiosperm plant shows at least one yield-related heterotic phenotype that is superior compared to a homozygous angiosperm plant having two wild-type alleles of said SFT gene.
2. The method of claim 1 , wherein the parent inbred angiosperm plant, homozygous for wild-type alleles of the gene, is isogenic to the mutant parent inbred angiosperm plant.
3. The method of claim 1 , wherein the wild-type SFT gene comprises the nucleic acid sequence as set forth in SEQ ID NO:2.
4. The method of claim 1 , wherein the mutated sft gene encodes a protein having the amino acid sequence set forth in SEQ ID NO:3.
5. The method of claim 4 , wherein the mutated sft gene comprises the nucleic acid sequence as set forth in SEQ ID NO:4.
6. The method of claim 1 , wherein the angiosperm plant is a Solanum lycopersicum plant.
7. A hybrid angiosperm plant produced according to the method of claim 1 .
8. The method of claim 1 , wherein the mutated sft gene encodes a protein having the amino acid sequence set forth in SEQ ID NO:5.
9. The method of claim 1 , wherein the mutated sft gene encodes a protein having the amino acid sequence set forth in SEQ ID NO:7.
10. The method of claim 8 , wherein the mutated sft gene comprises the nucleic acid sequence as set forth in SEQ ID NO:6.
11. The method of claim 9 , wherein the mutated sft gene comprises the nucleic acid sequence as set forth in SEQ ID NO:8.
12. A hybrid angiosperm plant comprising a wild-type SFT allele having the nucleic acid sequence set forth in SEQ ID NO:2 and a mutated sft allele having the nucleic acid sequence set forth in any one of SEQ ID NO:4, SEQ ID NO:6, and SEQ ID NO:8 wherein the mutation in the sft allele is such that it reduces SFT gene function when homozygous, and wherein said hybrid angiosperm plant shows at least one heterotic yield phenotype compared to a homozygous angiosperm plant having two wild-type alleles of the SET gene.
13. The hybrid angiosperm plant of claim 12 , wherein the mutant sft gene comprises a nucleic acid sequence as set forth in SEQ ID NO:4.