Tissue extraction and collection device
Methods and device for extracting and collecting tissue, which can be used for example in tissue engineering and grafting applications, are disclosed. In one embodiment, a device can include an outer tube. A rotatable shaft can be disposed within the outer tube can have a tissue harvesting tip formed on its distal end, the tissue harvesting tip being effective to excise tissue upon rotation thereof. A tissue collection device can be included to receive and collected excised tissue, and the tissue collection device can indicate the amount of tissue collected therein. For example, the tissue collection device can include a straining element which collects excised tissue and an indicator by which to assess the amount of collected tissue. In some embodiments, the tissue collection device can translate to indicate the amount of collected tissue. In many cases, devices disclosed herein can include driving mechanisms that are adapted to drive a tissue harvesting tip such that the tip excises soft tissue, but stops when contacting bone (or soon after contacting bone). In some embodiments, the tissue harvesting tip can be effective to excise viable tissue samples, such that the samples can exhibit desirable proportions of viable cells. Further, in some embodiments, the tissue harvesting tips can excise a tissue sample with tissue particles falling in certain size ranges.
1. A method for extracting and collecting tissue, comprising:
excising tissue by rotating a rotatable tissue harvesting tip formed at a distal end of a rotatable shaft;
transporting the excised tissue to a tissue collection device via a vacuum force; and
collecting the excised tissue in a tissue collection chamber, collection of the excised tissue causing linear sliding movement of the tissue collection chamber within a container extending between an inlet port and an outlet port of the tissue collection device along a longitudinal axis extending between the inlet port and the outlet port, wherein the tissue collection chamber includes a first portion that is sized to prevent tissue and fluid from passing between the tissue collection chamber and a sidewall of the container, as well as a second portion that is proximal to the first portion and allows fluid to pass through the tissue collection chamber while retaining the excised tissue, and wherein displacement of the tissue collection chamber indicates an amount of the excised tissue collected in the tissue collection chamber.
2. The method of claim 1 , wherein one or more indicators on the tissue collection device indicate displacement of the tissue collection chamber of the tissue collection device.
3. The method of claim 2 , wherein the one or more indicators are disposed on a transparent portion of the tissue collection device and indicate a degree of displacement of the tissue collection chamber which corresponds to the amount of tissue in the tissue collection chamber.
4. The method of claim 1 , further comprising removing excised tissue from the tissue collection device.
5. The method of claim 4 , wherein removing tissue comprises directing a flow of fluid through the tissue collection device.
6. The method of claim 1 , further comprising depositing excised tissue onto a tissue scaffold.
7. A method for extracting and collecting tissue, comprising:
excising tissue with a rotatable tissue harvesting tip formed at a distal end of a rotatable shaft; and
transporting the excised tissue to a tissue collection device via a vacuum force;
wherein the excised tissue causes movement of the tissue collection device along a longitudinal axis of the tissue collection device against a bias of a spring, and such movement indicates an amount of the excised tissue collected in the tissue collection device; and
wherein the tissue collection device allows fluid to pass therethrough while retaining the excised tissue.