Cellular extracts
The invention describes methods and agents for improving cosmetic appearance, for promoting, improving or restoring health of cells and tissues, preferably skin, and more preferably, for restoring aged or damaged skin to a healthy appearance. The agents include compositions of cells, eggs, cell extracts, egg extracts, and extract components such as purified nucleic acids, polypeptides, lipids, carbohydrates or other natural products.
1. A method of increasing collagen protein production and/or collagen gene expression by human skin fibroblasts of a subject, comprising:
applying a formulation containing from about 1% to about 5% volume/volume of a salmon egg cellular extract formulated in a pharmaceutically acceptable carrier—to the skin of said subject under conditions such that collagen protein production and/or collagen gene expression is increased in human skin fibroblasts at the site of application, wherein said salmon egg extract comprises about 180 to 260 mg/ml fish egg protein, from about 3.7 to 4.5 grams/100 grams extract fish egg lipids, an osmolarity of from about 330 to 440 mOsm, and a pH of from about 6.5 to 7.0.
2. The method of claim 1 , wherein said formulation is selected from the group consisting of a cream, gel, emulsion, ointment, spray, powder and a lotion.
3. The method of claim 1 , wherein said formulation is an oil in water emulsion.
4. The method of claim 1 , wherein said formulation is a lotion.
5. The method of claim 1 , wherein said formulation is an emulsion.
6. The method of claim 1 , wherein said formulation is a gel.
7. The method of claim 1 , wherein said formulation further comprises an emulsifying agent.
8. The method of claim 1 , wherein said formulation comprises an agent selected from the group consisting of fixed oil, mineral oil petrolatum, wax, hydrophilic colloid and combinations thereof.
9. The method of claim 1 , wherein said formulation comprises a gelling agent.
10. The method of claim 1 , wherein said formulation comprises a synthetic surfactant.
11. The method of claim 1 , wherein said formulation comprises a lipid agent from a source other than salmon.
12. The method of claim 11 , wherein said lipid agent is selected from the group consisting of castor-oil, olive oil, cacao oil, camellia oil, coconut oil, wood wax, jojoba oil, grape seed oil and avocado oil, mink oil, egg yolk oil, beeswax, whale wax, lanolin, carnauba wax, candelilla wax, liquid paraffin, squalene, microcrystalline wax, ceresine wax, paraffin wax, Vaseline, lauric acid, myristic acid, stearic acid, oleic acid, isostearic acid behenic acid; isopropyl myristate, isopropyl palmitate, octyldodecyl myristate, octyldodecyl oleate and cholesterol oleate.
13. The method of claim 1 , wherein said formulation comprises an alcohol.
14. The method of claim 13 , wherein said alcohol is selected from the group consisting of cetanol, stearyl alcohol, hexyldecanol, octyldecanol and lauryl alcohol.
15. The method of claim 1 , wherein said formulation further comprises a polyhydric alcohol.
16. The method of claim 15 , wherein said polyhydric alcohol is selected from the group consisting of ethylene glycol, polyethylene glycol, propylene glycol, 1,3-butyrene glycol, 1,4-butyrene glycol, dipropylene glycol, glycerol, diglycerol, triglycerol, tetraglycerol and other polyglycerols, glucose, maltose, maltitose, sucrose, fructose, xylitose, sorbitol, maltotriose, threitol and erythritol.
17. The method of claim 1 , wherein said formulation comprises a thickening agent.
18. The method of claim 17 , wherein said thickening agent is selected from the group consisting of sodium alginate, xanthene gum, aluminum silicate, quince seed extract, gum tragacanth, starch, collagen and sodium hyaluronate; semi-synthetic high molecular substances such as methyl cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, soluble starch and cationized cellulose; and synthetic high molecular substances such as carboxyvinyl polymer and polyvinyl alcohol.
19. The method of claim 1 , wherein said formulation further comprises a preservative.
20. The method of claim 19 , wherein said preservative is selected from the group consisting of a benzoate, a salicylate, a sorbate, a dehydroacetate, a p-oxybenzoate, 2,4,4′-trichloro-2′-hydroxydiphenyl ether, 3,4,4′-trichlorocarbanilide, benzalkonium chloride, hinokitiol, resorcinol and ethanol.
21. The method of claim 1 , wherein said formulation comprises a chelating agent.
22. The method of claim 1 , wherein said formulation comprises a UV absorbent.
23. The method of claim 22 , wherein said UV absorbent is selected from the group consisting of p-aminobenzoic acid, 2-ethoxyethyl p-methoxycinnamate, isopropyl p-methoxycinnamate, butylmethoxybenzoylmethane, glyceryl-mono-2-ethylhexanoyl-di-p-methoxybenzophenone, digalloyl trioleate, 2,2′-dihydroxy-4-methoxybenzophenone, ethyl-4-bishydroxypropylaminobenzoate, 2-ethylhexyl-2-cyano-3,3′-diphenyl acrylate, ethylhexyl p-methoxycinnamate, 2-ethylhexyl salicylate, glyceryl p-aminobenzoate, homomethyl salicylate, methyl o-aminobenzoate, 2-hydroxy-4-methoxybenzophenone, amyl p-dimethylaminobenzoate, 2-phenylbenzoimidazole-5-sulfonic acid and 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid.