Method for controlled release of parathyroid hormone from cross-linked hyaluronic acid hydrogel
A method for locally controlled release of an effective amount of PTH(1-34) by a hyaluronic acid based hydrogel that can injected intra-articularly for the treatment of osteoarthritis is provided.
1. A method for controlled release of parathyroid hormone (1-34) (PTH (1-34)) in a subject suffering from osteoarthritis, comprising:
(a) providing a hydrogel consisting of a UV-irradiated cross-linked methacrylated-hyaluronic acid (CLMH) and PTH (1-34) in acid solution; and
(b) administering the hydrogel to a joint of a subject suffering from osteoarthritis, wherein the hydrogel controls sustained-release of an effective amount of PTH (1-34).
2. The method of claim 1 , wherein the osteoarthritis is caused by terminal differentiation of chondrocytes.
3. The method of claim 1 , wherein the joint is a knee joint, hip joint, spine joint or finger joint.
4. The method of claim 1 , wherein the UV-irradiated cross-linked methacrylated-hyaluronic acid encapsulates the PTH (1-34).
5. The method of claim 1 , wherein the hydrogel has a three dimensional structure.
6. The method of claim 1 , wherein the effective amount is from about 2×10 −9 M to about 5×10 −8 M.
7. The method of claim 1 , wherein the effective amount is from about 4.3×10 −9 M to about 1.23×10 −8 M.
8. The method of claim 1 , wherein the administration is intra-articular injection.
9. The method of claim 1 , wherein the subject is a mammal.
10. The method of claim 9 , wherein the mammal is a human.
11. The method of claim 1 , wherein the effective amount is from about 1×10 −9 M to about 5×10 −7 M.