Anti-microbial compositions
Antimicrobial compounds from Epicoccum purpurascens (syn. E. negrum ) for use against plant and fungi pathogens. An orange yellow metabolite was isolated, the structure elucidated as a small group of compounds Epipyrone A-C and fungicidal activity demonstrated against plant pathogens, for example Botrytis cinerea and Lecanicillium muscarium . Agricultural and pharmaceutical compositions are provided, and use thereof in treating microbial infections in an animal or plant are also provided.
1. A method of removing, inhibiting, treating or ameliorating a microbial infection, the method comprising the step of contacting one or more microbes with an effective amount of a compound of formula (I):
or a salt, tautomer, stereoisomer, hydrate, or solvate thereof, wherein
R is selected from the group consisting of C-β-D-galactopyranose; C-α-D-galactofuranose; C-β-D-galactofuranose; C-β-L-galactopyranose; C-α-L-galactofuranose; C-β-L-galactofuranose; or a related pyranose.
2. The method of removing or inhibiting a microbial infection according to claim 1 , wherein the microbial infection is in a plant or plant part thereof, the method comprising the step of applying to the plant or plant part thereof a treatment effective amount of a compound of formula (I):
or a salt, tautomer, stereoisomer, hydrate, or solvate thereof, wherein
R is selected from the group consisting of C-β-D-galactopyranose; C-α-D-galactofuranose; C-β-D-galactofuranose; C-β-L-galactopyranose; C-α-L-galactofuranose; C-β-L-galactofuranose; or a related pyranose.
3. The method as claimed in claim 2 wherein the method also comprises the step of applying to the plant or plant part thereof a treatment effective amount of the compound to inhibit the growth of Botrytis or Lecanicillium.
4. The method as claimed in claim 2 wherein the plant part is a fruit or a vegetable.
5. The method of inhibiting, treating, or ameliorating a microbial infection according to claim 1 , which comprises administering to a subject in need of such inhibition, treatment, or amelioration an effective amount of a compound of formula (I):
or a salt, tautomer, stereoisomer, hydrate, or solvate thereof, wherein
R is selected from the group consisting of C-β-D-galactopyranose; C-α-D-galactofuranose; C-β-D-galactofuranose; C-β-L-galactopyranose; C-α-L-galactofuranose; C-γ-L-galactofuranose; or a related pyranose.
6. The method as claimed in claim 5 wherein the subject is a non-human animal subject.
7. The method as claimed in claim 5 wherein the compound is administered as a composition additionally comprising a pharmaceutically acceptable carrier or diluent.
8. The method as claimed in claim 7 wherein the subject is a non-human animal subject.
9. The method as claimed in claim 7 wherein the administration comprises topically administering the composition.
10. The method as claimed in claim 5 wherein the administration comprises topically administering the compound.
11. The method as claimed in claim 5 , wherein the compound of formula (I) is administered to the subject before the microbe infects the subject to thereby inhibit infection.