Ion generation using wetted porous material
The invention generally relates to systems and methods for mass spectrometry analysis of samples. In certain embodiments, the invention provides a mass spectrometry probe including at least one porous material connected to a high voltage source, in which the porous material is discrete from a flow of solvent.
1. A fluid dispenser, the dispenser comprising:
a fluid chamber, wherein walls of the fluid chamber do not comprise an internal standard; and
an outlet comprising a dissolvable material that comprises a set amount of an internal standard, wherein the outlet is positioned such that fluid introduced to the chamber must interact with the dissolvable material that comprises the internal standard prior to flowing through the outlet.
2. The dispenser according to claim 1 , wherein outlet is positioned at a bottom of the chamber.
3. The dispenser according to claim 1 , wherein the internal standard coats at least a portion of the dissolvable material.
4. The dispenser according to claim 1 , wherein the internal standard impregnates at least a portion of the dissolvable material.
5. The dispenser according to claim 1 , further comprising at least one overflow chamber.
6. A method for dispensing a sample, the method comprising:
providing a fluid dispenser comprising a fluid chamber, wherein walls of the fluid chamber do not comprise an internal standard; and an outlet comprising a dissolvable material that comprises a set amount of an internal standard, wherein the outlet is positioned such that fluid introduced to the chamber must interact with the dissolvable material that comprises the internal standard prior to flowing through the outlet;
introducing a sample to the dispenser;
interacting the sample with the internal standard for a time sufficient that the internal standard to be introduced into the sample; and
dispensing the sample through the outlet.
7. The method according to claim 6 , wherein outlet is positioned at a bottom of the chamber.
8. The method according to claim 6 , wherein the internal standard coats at least a portion of the dissolvable material.
9. The method according to claim 6 , wherein the internal standard impregnates at least a portion of the dissolvable material.
10. The method according to either claim 8 or 9 , wherein the sample causes the porous material to dissolve and the sample interacts with the internal standard as the porous material dissolves.
11. The method according to claim 6 , further comprising at least one overflow chamber.
12. The method according to claim 6 , wherein the sample is blood.