Methods for identifying fragile histidine triad (FHIT) interaction and uses thereof
Provided herein are methods and compositions for the diagnosis, prognosis and treatment of a cancer associated disorder using the Fhit gene.
1. A method for inducing an apoptosis process in a cell, comprising
exposing the cell to an exogenous fragile histidine triad (Fhit) gene product in an amount sufficient to cause generation of reactive oxygen species (ROS) in the cell; and
administering an oxidative stress to the cell, wherein the oxidative stress comprises peroxide, thereby inducing an apoptotic process in the cell.
2. A method for mediating an apoptotic process in at least one cell, comprising:
exposing the cell to a sufficient amount of an exogenous fragile histidine triad (Fhit) gene product to allow the Fhit to enter mitochondria in the cell and to interact with Fdxr protein in the cell, whereby the Fdxr resists proteosomal degradation and accumulates in the cell, thereby causing an increase in Fdxr protein level that is associated with generation of ROS, and
applying an oxidative stress to the cell, wherein the oxidative stress comprises peroxide, causing a change in the apoptotic process in the cell.
3. The method of claim 1 , further comprising:
administering at least one chemotherapeutic agent.
4. The method of claim 3 , wherein the at least one chemotherapeutic agent is selected from the group consisting of: paclitaxel and cisplatin.