Cell culture support for forming string-shaped cardiomyocyte aggregates
This invention is intended to provide cell aggregates that can reproduce functions of the myocardium, such as the function of beating, and thus are available for myocardial regenerative therapy, and to provide a cell culture support for producing the same. According to this invention, a cell culture support for forming string-shaped cardiomyocyte aggregates, which has a substrate portion having one surface on which at least one linear cell-adherent region is formed is provided.
1. A cell culture support for forming string-shaped cardiomyocyte aggregates comprising a substrate having:
a plurality of convex ridge portions, each having a linear, planar and reversibly cell-adherent upper surface, and
a plurality of linear concave grooves, wherein the inner surface of each groove is non-cell-adherent.
2. The cell culture support according to claim 1 , wherein the width of the linear, planar cell-adherent upper surface is 1 to 19 times wider than that of a cell to be cultured.
3. The cell culture support according to claim 1 , wherein the width of the linear cell-adherent upper surface is 5 to 40 μm.
4. The cell culture support according to claim 1 , wherein each of said linear convex ridge portions is separated from the other by one of the linear concave grooves.
5. The cell culture support according to claim 4 , wherein the distance between two adjacent linear convex ridge portions is at least about 20 μm.
6. The cell culture support according to claim 4 , wherein said concave groove is at least about 4 microns in depth.
7. The cell culture support according to claim 1 , wherein the linear planar cell-adherent upper surface of each convex ridge portion is coated with an environmentally-responsive high molecular compound that has cell adhesion properties under cardiomyocyte culture conditions.