Methods for using synthetic triterpenoids in the treatment of bone or cartilage diseases or conditions
The present invention features the use of a synthetic triterpenoid to induce gene expression and differentiation of stem or progenitor cells in the treatment of bone/cartilage diseases or conditions.
1. A method for producing a stem or progenitor cell with induced gene expression comprising contacting a stem or progenitor cell ex vivo with an effective amount of a synthetic triterpenoid to induce the expression of one or more of SOX9 (Sex determining region Y-box 9), COL2A1 (Type II Collagen (alpha1)), TGF (Transforming Growth Factor)-β1, TGF-β2, TGF-β33, BMP (Bone Morphogenic Protein) 2, BMP4, BMPRII (Bone Morphogenic Protein Receptor II), SMAD (Small Mothers Against Decapentaplegic) 3, SMAD4, SMAD6, SMAD7, TIMP (Tissue Inhibitor of Metalloproteinase)-1 or TIMP-2 in the stem or progenitor cell, wherein the stem or progenitor cell is a mesenchymal stem cell, adipose tissue-derived mesenchymal stem cell, embryonic stem cell, stem cell from exfoliated deciduous teeth, periosteum cell, osteoprogenitor cell, or growth plate progenitor cell and the synthetic triterpenoid is a compound having the structure:
2. The method of claim 1 , wherein the stem or progenitor cell is multipotent.