Method of efficiently establishing induced pluripotent stem cells
Provided are a method of improving the efficiency of establishment of iPS cells, comprising the step of contacting one or more substances selected from the group consisting of members of the GLIS family (e.g., GLIS1) and nucleic acids that encode the same and one or more substances selected from the group consisting of members of the Klf family and nucleic acids that encode the same, with a somatic cell, an iPS cell comprising an exogenous nucleic acid that encodes a member of the GLIS family or a member of the Klf family, that can be obtained by the method, and a method of producing a somatic cell by inducing the differentiation of the iPS cell.
1. A method of producing an iPS cell, comprising
culturing a somatic cell transfected with nucleic acids that encode the following (1), (2), (3), and (4):
(1) GLIS1,
(2) Oct3/4,
(3) a member of the Klf family selected from the group consisting of Klf1, Klf2, Klf4, and Klf5,
(4) a member of the Sox family selected from the group consisting of Sox1, Sox2, Sox3, Sox15, and Sox17,
thereby producing an iPS cell.
2. The method according to claim 1 , wherein the member of the Klf family is Klf4.
3. The method according to claim 1 , wherein the somatic cell has been transfected with a further nucleic acid that encodes a reprogramming substance selected from the group consisting of a member of the Myc family, a member of the Lin28 family, and Nanog,
wherein the member of the Myc family is selected from the group consisting of c-Myc, L-Myc and N-Myc, and
wherein the member of the Lin28 family is selected from the group consisting of Lin28 and Lin28b.
4. The method according to claim 1 , wherein the member of the Sox family is Sox2.
5. The method according to claim 1 , wherein the member of the Myc family is c-Myc.
6. A method of producing a somatic cell, comprising the following (1) and (2):
(1) the step of producing an iPS cell by the method according to claim 1 , and
(2) the step of treating the iPS cell obtained through the step (1) above to induce it to differentiate into a somatic cell.