Methods and systems for optical characterisation
An optical device is described for irradiating at least one object in a medium. The optical device may be a microfluidics device, and comprises at least one integrated planar waveguide that enables providing sheet irradiation of objects in the medium. A characterization system including such an optical device and a corresponding method of characterizing an object or a fluid are described.
1. An optical device for irradiating at least one object in a medium,
the optical device comprising at least one integrated planar waveguide arranged to provide sheet irradiation in a measurement region;
wherein the at least one integrated waveguide defining an irradiation sheet has a height in a first direction perpendicular to the propagation direction of radiation in the at least one integrated waveguide, the height being at least three times smaller than a width in a second direction perpendicular to the propagation direction of radiation in the at least one waveguide; and
wherein the optical device is configured for allowing detection of a response to the sheet irradiation in a direction perpendicular to the irradiation sheet.
2. The optical device according to claim 1 , the integrated waveguide being arranged to support a single laser mode.
3. The optical device according to claim 1 , wherein the device is configured to detect a response signal from the at least one object being irradiated in the measurement region.
4. The optical device according to claim 1 , said medium comprising a fluidic sample, the device furthermore comprising at least one transportation channel for transportation of the medium comprising the at least one object of interest, the transportation channel containing or being in contact with the planar waveguide such that a sheet of irradiation is generated within the channel or a part thereof forming the measurement region.
5. The optical device according to claim 4 , the transportation channel being an integrated microfluidics channel, wherein the optical device is an optofluidics device and the measurement region is part of the integrated microfluidics channel of the optofluidics device.
6. The optical device according to claim 4 , wherein the at least one integrated planar waveguide has a core defined by two major surfaces, wherein none of the major surfaces lies in plane with a bottom surface of the transportation channel.
7. The optical device according to claim 4 , wherein the optical device comprises a substrate layer, an integrated planar waveguide and a second component being attached to the substrate layer, and wherein the integrated planar waveguide and second component are arranged so that the transportation channel is substantially formed from the substrate layer, the integrated planar waveguide and the second component.
8. The optical device according to claim 1 , the optical device furthermore comprising electrodes providing an electric field in a direction perpendicular to a transportation direction of the at least one object of interest.
9. The optical device according to claim 1 , the optical device comprising a plurality of measurement regions, wherein the device is arranged to image a plurality of objects in different measurement regions.
10. The optical device according to claim 1 , wherein the optical device comprises two integrated waveguides at opposite sides of the measurement region, said waveguides being configured so that their sheet illumination coincides.
11. The optical device according to claim 1 , wherein the optical device comprises two integrated waveguides at opposite sides of the measurement region, said waveguides being configured so that their sheet illumination does not coincide.
12. A characterisation system for characterising at least one object in a medium, the characterisation system cooperating with or comprising an optical device according to claim 1 , the characterisation system comprising a radiation source generating a radiation beam irradiating the at least one object using said optical device and a detection system detecting a response due to interaction of the at least one object with the irradiation sheet generated using the optical device, wherein the detection system is configured to detect the signal in a direction perpendicular to an average plane through the irradiation sheet.
13. The characterisation system according to claim 12 , wherein the detection system is configured so that the focal plane of the detection system for capturing the detection signal coincides with the irradiation sheet.
14. The characterization system according to claim 12 , the system furthermore comprising coupling devices arranged to couple radiation into the waveguide of the optical device.
15. The characterization system according to claim 12 , wherein the radiation source is integrated in the optical device.
16. A method for characterising an object in a medium, the method comprising
generating sheet irradiation in a measurement region using an integrated planar waveguide, wherein the at least one integrated waveguide defines an irradiation sheet having a height in a first direction perpendicular to the propagation direction or radiation in the at least one integrated waveguide, the height being at least three times smaller than a width in a second direction perpendicular to the propagation direction of radiation in the at least one waveguide,
providing interaction between the sheet irradiation and at least one object to be characterised, and
detecting a signal responsive to interaction between the sheet irradiation and the object of interest in a direction perpendicular to the irradiation sheet.
17. The method according to claim 16 , wherein the method furthermore comprises coupling a radiation beam in the integrated planar waveguide.