Monoclonal antibody against D-dimer and diagnosis agent for detecting D-dimer, crosslinked fibrin and its derivatives containing D-dimer by using the antibody
Disclosed are a monoclonal antibody against human D-dimer produced in a mouse and high molecular weight cross-linked fibrin including a corresponding epitope, a cell line secreting the monoclonal antibody, and methods for producing the same. The anti-D-dimer monoclonal antibody of the present invention may be effectively used as a diagnotic agent for screening and detecting in-vivo D-dimer, and high molecular weight cross-linked fibrin and its derivatives containing the D-dimer since the monoclonal antibody specifically reacts with D-dimer, and cross-linked fibrin and its derivatives containing the D-dimer, which do not bind to human fibrinogen or fibrin.
1. A monoclonal antibody that binds to D-dimer and crosslinked fibrin, wherein the monoclonal antibody binds to a site comprising the amino acid sequence set forth in SEQ ID NO: 1.
2. A monoclonal antibody that binds to D-dimer and crosslinked fibrin, wherein the monoclonal antibody binds to a site comprising the amino acid sequence set forth in SEQ ID NO: 2.
3. A diagnostic construct for detecting D-dimer and crosslinked fibrin comprising:
(a) a first monoclonal antibody that binds to D-dimer and crosslinked fibrin, wherein the monoclonal antibody binds to a site comprising the amino acid sequence set forth in SEQ ID NO: 1 and
(b) a second monoclonal antibody that binds to D-dimer and crosslinked fibrin, wherein the second monoclonal antibody binds to a site comprising the amino acid sequence set forth in SEQ ID NO: 2 and said antibodies form a sandwich having a D-dimer or crosslinked fibrin molecule as a target analyte to be detected; and
(c) an optional substrate including a material for inducing color development by reacting with a marker.
4. The diagnostic construct for detecting D-dimer and crosslinked fibrin according to claim 3 , wherein the marker is colloidal gold sol, horseradish peroxidase (HRP) or alkaline phosphatase.
5. The diagnostic construct for detecting D-dimer and crosslinked fibrin according to claim 4 , wherein the substrate further includes a coupler selected from the group consisting of O-phenylenediamine and hydrogen peroxide.
6. A kit for detecting D-dimer comprising a monoclonal antibody that binds to a site comprising the amino acid sequence set forth in SEQ ID NO: 1 or the amino acid sequence set forth in SEQ ID NO: 2.
7. The kit according to claim 6 , wherein any one of said monoclonal antibodies are immobilized to a solid surface as a capture antibody and the other antibody is labeled with a detector probe wherein the detector probe is selected from a group consisting of metallic colored materials, non-metallic colored materials, chromogenic material, fluorochromic material, and other luminous material wherein the detector probe is suitable for visual or quantitative detection of D-dimer and is an aid for diagnosis of clinical symptoms associated with or resulting in the rise of the level of D-dimer in a patient's blood.
8. The monoclonal antibody according to claim 1 , wherein the monoclonal antibody binds with a site on a beta-chain of the D-dimer and the crosslinked fibrin.
9. The monoclonal antibody according to claim 2 wherein the antibody binds with a site on the alpha-chain of D-dimer and crosslinked.
10. A diagnostic construct for detecting D-dimer and crosslinked fibrin, comprising:
(a) a first monoclonal antibody as a primary antibody that binds with a site on a beta-chain of the D-dimer and the crosslinked fibrin;
(b) a second monoclonal antibody as a secondary antibody that binds with a site on an alpha-chain of D-dimer and crosslinked fibrin; and
(c) a substrate or detector probe including a material for inducing color development by reacting with a marker.
11. The diagnostic construct for detecting D-dimer and crosslinked fibrin according to claim 10 , wherein the marker is horseradish peroxidase (HRP) or alkaline phosphatase.
12. The diagnostic construct for detecting D-dimer and crosslinked fibrin according to claim 10 , wherein the substrate includes a coupler selected from the group consisting of o-phenylenediamine and hydrogen peroxide.
13. A kit for detecting a rise in D-dimer level in blood comprising a first monoclonal antibody that is immobilized on a solid surface as a capturing agent that binds to a site comprising the amino acid sequence set forth in SEQ ID NO: 1 and a second antibody that binds to a site comprising the amino acid sequence set forth in SEQ ID NO: 2, said antibodies are independently labeled with a detector probe, wherein the detector probe is selected from a group consisting of metallic colored materials, non-metalic colored materials, chromogenic material, fluorochromic material, and other luminous material wherein the detector probe is suitable for visual or quantitative detection of D-dimer.
14. A kit for detecting clinical symptoms of thrombosis and embolism comprising: at least one monoclonal antibody that is immobilized on a solid surface as a capturing agent that binds to a site comprising the amino acid sequence set forth in SEQ ID NO: 1 or in SEQ ID NO: 2, wherein the monoclonal antibody is used in a competitive assay or in sandwich immunoassay for the detection and quantitation of D-dimer and is an aid for diagnosing of clinical symptoms associated with or resulting in increased levels of D-dimer in a patient's blood.
15. A hybridoma that produces a monoclonal antibody or fragment thereof wherein the monoclonal antibody binds to a site comprising the amino acid sequence set forth in SEQ ID NO: 1 or 2.
16. A diagnostic method for detecting a rise in plasma concentration of D-dimer and crosslinked fibrin comprising:
(a) applying a first monoclonal antibody that binds to a region of D-dimer or crosslinked fibrin molecule comprising an amino acid sequence set forth in SEQ ID NO: 1 to a sample obtained from a patient in need thereof,
(b) applying a second monoclonal antibody that binds to a region of D-dimer or crosslinked fibrin molecule comprising amino acid sequence set forth in SEQ ID NO: 2 to said sample,
(c) forming at least one immunocomplex between at least one of said antibodies and the D-dimer or crosslinked fibrin molecule as a target analyte,
(d) quantitatively detecting the presence or absence of said target analyte using a detector probe which binds to the immunocomplex, wherein an increase in the quantitative detection of said probe signifies a rise in plasma concentration of the target analyte.
17. The method of claim 16 , further comprising the step of reacting a marker enzyme to the detector probe to induce color development.
18. The method of claim 17 , wherein the marker is horseradish peroxidase (HRP) or alkaline phosphatase.
19. The method of claim 16 , wherein the rise in the plasma concentration of target analyte the presence of a pathological condition secondary to activated clotting factor.
20. The method of claim 16 , wherein said detector probe is selected from a group consisting of metallic colored materials, non-metalic colored materials, chromogenic material, fluorochromic material.
21. The method of claim 16 , further comprising the step of immobilizing the first monoclonal antibody on a solid surface.
22. The method of claim 16 , further comprising the step of reacting the first monoclonal antibody with a plasma sample containing the D-dimer targeting analyte.
23. The method of claim 16 , wherein said sample is a blood or a tissue sample.