Method for distinguishing and sorting of cells and device therefor
A method for distinguishing and sorting cells characterized by comprising distinguishing and sorting a specific cell mass or a part of the cells in the cell mass with the use of transmitted light data reflecting the morphological characteristics of the cells such as size and shape optionally together with side-scattering light data reflecting the characteristics of the internal structure of the cells. The part of the cells in the specific cell mass as described above are at the G1 stage or at a part of the M stage in the cell cycle. A part of the cells at the G1 stage are referred to as the left bottom line in an analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data, while a part of the cells at the M stage are referred to as the right bottom line in the analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data.
1. A method of distinguishing and sorting cells, comprising:
irradiating living cells with light;
receiving transmitted light that is transmitted through the living cells;
processing the transmitted light to obtain transmitted light information;
receiving at least one of side-scattering light and fluorescent light from the living cells that are irradiated with light;
processing the at least one of the side-scattering light and the fluorescent light to obtain one or both of side-scatter light information and fluorescent light information; and
distinguishing and sorting a specific cell mass or a part of the living cells in the specific cell mass by using the transmitted light information and one or both of the side-scattering light information and the fluorescent light information, wherein one or both of the side-scattering light information and the fluorescent light information indicates the characteristics of the internal structures of the living cells, and the transmitted light information indicates at least one of: (a) morphological characteristics of the living cells, including at least one of size and shape of the living cells, and (b) characteristics of internal structures of the living cells,
wherein the specific cell mass or the part of the living cells in the specific cell mass is distinguished and sorted, regardless of a presence of fluorescent processing that includes an antibody-antigen reaction at a cell surface and a presence of an expression of fluorescent protein in a cell being performed on the part of the living cells in the specific cell mass,
the part of the living cells in the specific mass includes stem cells and cells at an S phase of the cycle are distinguished using the transmitted light information, and
the stem cells that are bigger than a biggest cell at the S phase are sorted by using a histogram of cells where an abscissa axis indicates the transmitted light information, or by using an analytical dispersion diagram of cells where an abscissa axis indicates the transmitted light information and a vertical axis indicates the side-scattering light information, the transmitted light information, or the fluorescent light information.
2. The method of distinguishing and sorting cells according to claim 1 , further comprising:
sorting one cell from the part of the living cells in the specific cell mass in one well.
3. The method of distinguishing and sorting cells according to claim 1 , wherein the part of the living cells in the specific cell mass is a part of the living cells at a G1phase of a cell cycle.
4. A method of distinguishing and sorting cells, comprising:
irradiating living cells with light;
receiving transmitted light that is transmitted through the living cells;
processing the transmitted light to obtain transmitted light information; and
distinguishing and sorting a specific cell mass or a part of the living cells in the specific cell mass by using the transmitted light information, the transmitted light information indicating at least one of: (a) morphological characteristics of the living cells, including at least one of size and shape of the living cells, and (b) characteristics of internal structures of the living cells,
wherein the specific cell mass or the part of the living cells in the specific cell mass is distinguished and sorted, regardless of a presence of fluorescent processing that includes an antibody•antigen reaction at a cell surface and a presence of an expression of fluorescent protein in a cell being performed on the part of the living cells in the specific cell mass,
the part of the living cells in the specific mass includes stem cells and cells at an S phase of the cycle are distinguished using the transmitted light information, and
the stem cells that are bigger than a biggest cell at the S phase are sorted by using a histogram of cells where an abscissa axis indicates the transmitted light information.
5. The method of distinguishing and sorting cells according to claim 4 , further comprising:
sorting one cell from the part of the living cells in the specific cell mass in one well.