Microwell device
A microwell device is provided. The device includes a plate having a upper surface. The upper surface has first and second recesses formed therein. Each recess has an outer periphery. First and second portions of microwells are formed in upper surface of the plate. The first portion of microwells are spaced about the outer periphery of the first recess and the second portion of microwells spaced about the outer periphery of the first recess. A first barrier is about a first portions of the microwells for fluidicly isolating the first portion of the microwells and a second barrier about a second portions of microwells for fluidicly isolating the second portion of the microwells.
1. A microwell device, comprising:
a plate having a upper surface including a plurality of microwells formed therein, each of the microwells having a volume and being adapted for receiving a fluid therein; and
a barrier extending about a first portion of the microwells, the barrier preventing fluid deposited on the first portion of the microwells from flowing therepast, wherein the barrier is a channel formed in the upper surface of the plate; and
a recess formed in the upper surface of the plate within the barrier, the recess being fluidicly isolated from the barrier and having a volume greater than the volume of each of the microwells.
2. The device of claim 1 wherein the recess has an outer periphery and wherein the first portion of microwells are spaced about the outer periphery of the recess.
3. The device of claim 1 wherein the channel has a volume and wherein each of the microwells has a volume, the volume of the channel being greater than the volumes of the microwells.
4. The device of claim 1 wherein the barrier is generally circular.
5. The device of claim 1 wherein the barrier is a first barrier and wherein the device further comprises a second barrier extending about a second portion of the microwells, the second barrier preventing fluid deposited on the second portion of the microwells from flowing therepast.
6. A microwell device, comprising:
a plate having a upper surface including a plurality of microwells formed therein, each of the microwells having a volume and being adapted for receiving a fluid therein;
first and second recesses formed in the upper surface of the plate, each recess having a volume and an outer periphery;
a first barrier extending about the first recess and positioned between the first and second portions of microwells for fluidicly isolating the first portion of the microwells from the second portion of microwells, the first barrier being fluidicly isolated from the first recess, wherein the first barrier includes a first channel extending about the first portion of microwells in the upper surface of the plate;
a second barrier extending about the second recess and positioned between the first and second portions of microwells for fluidicly isolating the second portion of the microwells from the first portion of microwells, the second barrier being fluidicly isolated from the second recess; and
wherein:
the volume of each of the first and second recesses is greater than the volume of each of the microwells;
a first portion of microwells is spaced about the outer periphery of the first recess; and
a second portion of microwells is spaced about the outer periphery of the second recess.
7. The device of claim 6 wherein the first channel has a generally circular configuration.
8. The device of claim 6 wherein the first channel has a volume, the volume of the first channel being greater than the volumes of each of the first portion of microwells.
9. The device of claim 6 wherein the second barrier includes a second channel extending about the second portion of microwells in the upper surface of the plate.
10. A microwell device, comprising:
a plate having a upper surface, the upper surface including:
first and second recesses formed in the upper surface of the plate, each recess having an outer periphery and a volume;
a first portion of microwells formed therein, the first portion of microwells spaced about the outer periphery of the first recess and each of the first portion of microwells having a volume less than the volume of the first recess;
a second portion of microwells formed therein, the second portion of microwells spaced about the outer periphery of the second recess and each of the second portion of microwells having a volume less than the volume of the second recess;
a first barrier about the first recess and the first portion of the microwells for fluidicly isolating the first portion of the microwells, the first barrier being fluidicly isolated from the first recess, wherein the first barrier includes a first channel extending about the first portion of microwells; and
a second barrier about the second recess and the second portion of microwells for fluidicly isolating the second portion of the microwells, the second barrier being fluidicly isolated from the second recess.
11. The device of claim 10 wherein the first channel has a generally circular configuration.
12. The device of claim 10 wherein the first channel has a volume, the volume of the first channel being greater than the volumes of each of the first portion of microwells.
13. The device of claim 10 wherein the second barrier includes a second channel extending about the second portion of microwells.
14. The device of claim 10 further comprising a lid having a surface, the lid moveable between a first position wherein the surface of the lid is spaced from the upper surface of the plate and a second position wherein the surface of the lid is in engagement with the upper surface of the plate.