Method of producing and applications of composition of hypochlorous acid
A method for preparing a stabilized antimicrobial hypochlorous acid solution by diluting an aged stock solution to provide a hypochlorous acid solution at a concentration of about 50 to about 7000 ppm at a pH range of about 2.8 to about 4.0. The antimicrobial hypochlorous acid solution maintains at least 75 percent of the available chlorine present over a period of about 6 months to about 12 months. The antimicrobial hypochlorous acid solution has medical applications in humans and veterinary practice, both prophylactic and therapeutic. The solution can also be used for non-medical applications in antisepsis and sterilization of surfaces.
1. A method of preparing a stabilized composition of an antimicrobial hypochlorous acid solution comprising the steps of:
a) providing an aqueous stock solution that contains an admixture of about 18 percent to about 22 percent solution A, about 1.0 percent to about 1.4 percent solution B, and about 70 percent to about 86 percent solution C,
wherein said solution A contains about 13 percent sodium hypochlorite, said solution B contains about 33 percent hydrochloric acid, and said solution C is water,
b) admixing said solution C and said solution A to yield solution E, wherein said solution E contains about 2 to about 3 percent sodium hypochlorite,
c) forming said aqueous stock solution by admixing said solution E with said solution B, wherein said aqueous stock solution contains about 0.3 percent to about 0.5 percent hydrogen chloride,
wherein said aqueous stock solution has (i) a pH value of about 4 to about 6, and (ii) an oxidation reduction potential of about 1100 m.v. to about 1450 m.v., said aqueous stock solution being maintained at ambient temperature in a sealed container in the dark for about 18 to about 30 hours to form an aged stock solution; and
d) forming said antimicrobial hypochlorous acid solution by i) diluting said aged stock solution to provide a hypochlorous acid solution at a concentration of about 1500 to about 3000 ppm i) adjusting the pH value to about 2.8 to about 4.0, said antimicrobial hypochlorous acid solution, when sealed and in the dark (in the dark) at a temperature of about 18 to about 22° C., maintaining at least about 75 percent of the available chlorine present as hypochlorous acid over a period of about 6 months to about 12 months, wherein available chlorine present is first measured at a time within about 24 hours after formation of the antimicrobial hypochlorous acid solution.
2. The method of claim 1 , further comprising the steps to form a salified antimicrobial hypochlorous acid solution:
b′) admixing said solution E with solution D to form a salified solution E, said solution D containing 0.006 percent to about 0.008 percent sodium chloride,
b″) forming salified aqueous stock solution by admixing the salified solution E with said solution B,
wherein said salified aqueous stock solution has (i) a pH value of about 4 to about 6, (ii) a conductivity of about 22 ds/m to about 27 ds/m., and (iii) an oxidation reduction potential of about 1100 m.v. to about 1450 m.v., said aqueous stock solution being maintained at ambient temperature in a sealed container in the dark for about 18 to about 30 hours to form an aged salified stock solution; and
d) forming said salified antimicrobial hypochlorous acid solution by i) diluting said salified aged stock solution to provide a salified hypochlorous acid solution at a concentration of about 1500 to about 3000 ppm and ii) adjusting the pH value to about 2.8 to about 4.0, said salified antimicrobial hypochlorous acid solution when sealed and in the dark at a temperature of about 18 to about 22° C. maintains at least about 75 percent of the available chlorine present over a period of about 6 months to about 12 months, wherein said available chlorine present as hypochlorous acid is first measured at a time after formation of said salified aged stock solution.