Method and kits to detect beryllium by fluorescence
A low-cost practical method of determining beryllium or a beryllium compound thereof in a sample is disclosed by measuring fluorescence. This method discloses optical filters for use with low cost fluorometers which may be used to quantitatively determine the presence of beryllium. This method may be extended to estimate particle size distribution for particles comprising Beryllium. A method is also disclosed to store solutions for extended period of time so that these materials are stable during transportation and in the labs.
1. A method of determining the presence or amount of beryllium in a quantity lower than 0.02 ug in a sample, comprising:
admixing a sample suspected of containing beryllium or a beryllium compound with a dissolution solution whereby beryllium or a beryllium compound within said sample is dissolved;
mixing a portion from said admixture with a buffered solution comprising a fluorescent indicator capable of binding beryllium or a beryllium compound to the fluorescent indicator; wherein the volumetric ratio of the said admixture and the buffered solution is about 1:4, and the pH of the resulting solution is at least 12, and determining the presence or amount of beryllium or a beryllium compound within said sample by measuring fluorescence from said fluorescent indicator;
wherein the fluorescence signal is detected by passing through an optical filter which has a peak transmission in the range of 440 nm to 500 nm with a minimum bandwidth of 5 nm.
2. The method of claim 1 , wherein the dissolution solution comprises at least one of ammonium bifluoride solution and an acid.