Targeted sequencing of biomolecules by pulling through a liquid-liquid interface with an atomic force microscope
View Patent ↗A mechanism is provided for sequencing a biopolymer. The biopolymer is traversed from a first medium to a second medium. The biopolymer includes bases. As the biopolymer traverses from the first medium to the second medium, different forces are measured corresponding to each of the bases. The bases are distinguished from one another according to the different measured forces which are measured for each of the bases.
1. An apparatus for sequencing a block copolymer, the apparatus comprising:
a chamber filled with a first medium and a second medium; and
an atomic force microscope;
wherein the atomic force microscope is configured to:
traverse the block copolymer from the first medium to the second medium, wherein the block copolymer comprises first blocks and second blocks;
measure a first measured force when the first blocks traverse from the first medium to the second medium;
measure a second measured force when the second blocks traverse from the first medium to the second medium, the first measured force being different from the second measured force;
identify the first blocks of the block copolymer based on measuring the first measured force; and
identify the second blocks of the block copolymer based on measuring the second measured force.
2. The apparatus of claim 1 , wherein the first measured force is a peak when the first blocks respectively traverse from the first medium to the second medium; and
wherein the second measured force is a valley when the second blocks respectively traverse from the first medium to the second medium.
3. The apparatus of claim 1 , wherein the first medium is water; and
wherein the second medium is oil.