Acid ceramidase and mammalian cell survival
The present invention relates to methods of promoting the survival of cells by treating the cells with acid ceramidase. A kit for promoting ex vivo cell survival is also disclosed, as is a method of predicting in vitro fertilization outcome of a female subject.
1. A method of promoting the ex vivo survival of mammalian cells, said method comprising:
providing one or more mammalian cells ex vivo, wherein the one or more mammalian cells are selected from the group consisting of eggs, primary cells, neurons, sperm, synovial fibroblasts, and embryonic stem cells; and
treating the one or more mammalian cells with acid ceramidase under conditions effective to promote survival of the one or more mammalian cells.
2. The method of claim 1 , wherein the one or more mammalian cells are provided in a culture medium and the acid ceramidase is added to the culture medium during said treating.
3. The method of claim 1 , wherein the one or more mammalian cells are one or more eggs.
4. The method of claim 3 , wherein the one or more eggs are unfertilized.
5. The method of claim 3 , wherein the one or more eggs are fertilized.
6. The method of claim 3 , wherein the one or more eggs are from a human.
7. The method of claim 1 , wherein said treating is carried out with acid ceramidase in protein form.