Membrane-coated particles
A membrane-coated particle composition and methods comprising a particle surrounded by a native cell membrane are disclosed. The cell membrane may contain selected receptors or binding components. At least a portion of the receptors or binding components are oriented on the membrane-coated particle in the same or similar orientation as in the native cell membrane. The membrane-coated particle(s) finds use, for example, in contexts of basic research, proteomics, drug discovery, drug delivery, medical diagnostics, and aspects of patient care.
1. A composition comprising:
a particle having an exterior surface,
a coating comprising membrane portions of lysed whole cells at least partially surrounding the particle; and
at least one component associated with the membrane portions, the component having a binding affinity for a ligand upon contact with the component;
wherein at least 5% of said at least one component is in the same external orientation in the coating as in the whole cells; and
wherein the membrane portions are fluidly localized above the particle exterior surface.
2. The composition of claim 1 , wherein at least 90% of the at least one component is in the same external orientation as that of a native cell membrane.
3. The composition of claim 1 , wherein at least 80% of the at least one component is in the same external orientation of that of a native cell membrane.
4. The composition of claim 1 , wherein at least 70%, 60%, 50%, 40%, 30%, 20%, or 10% of the at least one component is in the same external orientation as a native cell.
5. The composition of claim 1 , wherein the particle is spherical.
6. The composition of claim 1 , wherein the particle comprises a material selected from the group consisting of silica, glass, borosilicate glass, polystyrene, polymethylmethacrylate, metal, semiconducting material, and organic material.
7. The composition of claim 1 , wherein the particle comprises a material that is non-porous, porous, or mesoporous.
8. The composition of claim 1 , wherein the particle is doped with a positively or negatively charged ion.
9. The composition of claim 1 , wherein the particle is coated with functional amine silanes.
10. The composition of claim 1 , wherein the membrane portions are selected from the group consisting of extracellular faces of an extracellular membrane, cytoplasmic faces of the extracellular membrane, and intracellular membranes.
11. The composition of claim 1 , wherein the membrane portions comprise a single bilayer.
12. The composition of claim 1 , wherein the component is selected from the group consisting of a protein, a lipid, and a carbohydrate.
13. The composition of claim 1 , wherein the component has binding affinity for the ligand, which is selected from the group consisting of a small molecule, a biomolecule, a peptide, a protein, an antibody, a plasma membrane vesicle, a cell surface, a liposome, a lipid, and a carbohydrate.
14. A system comprising a plurality of the compositions of claim 1 .