Isotachophoresis having interacting anionic and cationic shock waves
Improved electrophoretic analysis is provided by interaction of anionic and cationic isotachophoresis (ITP) shock waves that propagate toward each other, and analysis of the resulting interaction zones. These shock wave interactions can provide qualitatively different capabilities from conventional ITP methods. Shock wave interaction can enable a single assay to identify analyte and quantify its concentration via isotachophoretic focusing followed by separation of the concentrated analytes via electrophoresis, without any mid-assay alteration of the externally imposed experimental conditions (i.e., no switching, valve operation, etc. during the measurement). As another example, shock wave interaction can enable a single assay to provide coupled ITP processes with different electrolyte concentrations (as in cascade-ITP) in a single simple system (again, without any mid-assay alteration of the externally imposed experimental conditions).
1. A method comprising:
establishing anionic isotachophoresis (ITP) having one or more anionic ITP shock waves between an anionic leading electrolyte ion (LE−) and an anionic trailing electrolyte ion (TE−) in a channel;
establishing cationic isotachophoresis having one or more cationic ITP shock waves between a cationic leading ion (LE+) and a cationic trailing ion (TE+) in the channel;
selecting ions of the anionic ITP and the cationic ITP such that an interaction of the anionic ITP shock waves and cationic ITP shock waves modifies the effective mobility of one or more ions to provide a focusing-to-separation operation mode;
wherein the anionic ITP shock waves and the cationic ITP shock waves propagate toward each other and interact when they meet;
wherein one or more interaction regions are created by shock wave interaction of one or more of the anionic ITP shock waves with one or more of the cationic ITP shock waves; and
analyzing at least one of the interaction regions to determine physicochemical properties of ions and/or interaction regions in the channel;
further comprising isotachophoretic focusing of one or more analytes in a selected shock wave of the anionic and cationic ITP shock waves prior to the shock wave interaction; and
further comprising electrophoretic separation of the one or more analytes in at least one of the interaction regions after the shock wave interaction, wherein shock wave interaction causes an automatic transition from an ITP focusing mode to an electrophoretic separation mode.
2. A method comprising:
establishing anionic isotachophoresis (ITP) having one or more anionic ITP shock waves between an anionic leading electrolyte ion (LE−) and an anionic trailing electrolyte ion (TE−) in a channel;
establishing cationic isotachophoresis having one or more cationic ITP shock waves between a cationic leading ion (LE+) and a cationic trailing ion (TE+) in the channel;
selecting ions of the anionic ITP and the cationic ITP such that an interaction of the anionic ITP shock waves and cationic ITP shock waves modifies the effective mobility of one or more ions to provide a focusing-to-focusing operation mode;
wherein the anionic ITP shock waves and the cationic ITP shock waves propagate toward each other and interact when they meet;
wherein one or more interaction regions are created by shock wave interaction of one or more of the anionic ITP shock waves with one or more of the cationic ITP shock waves; and
analyzing at least one of the interaction regions to determine physicochemical properties of ions and/or interaction regions in the channel;
further comprising isotachophoretic focusing of one or more analytes in a selected shock wave of the anionic and cationic ITP shock waves prior to the shock wave interaction; and
further comprising isotachophoretic focusing of the one or more analytes in at least one of the interaction regions after the shock wave interaction, wherein shock wave interaction causes an automatic transition from an ITP focusing mode to another ITP focusing mode.
3. The method of claim 2 , further comprising performing the method of claim 2 such that ITP zone lengths of analytes in at least one of the interaction regions differ from ITP zone lengths of the analytes prior to the shock wave interaction.
4. The method of claim 3 , further comprising performing the method of claim 3 such that ITP zone lengths of analytes in at least one of the interaction regions are greater than ITP zone lengths of the analytes prior to the shock wave interaction.
5. The method of claim 1 , further comprising performing the method of claim 1 such that the anionic ITP has two or more anionic ITP zones prior to the shock wave interaction, such that the cationic ITP has two or more cationic ITP zones prior to the shock wave interaction, and such that at least one of the interaction regions has a composition that is distinct from compositions of the anionic and cationic ITP zones.
6. The method of claim 1 , further comprising extracting one or more of the interaction regions from the channel for use and/or further analysis.
7. The method of claim 1 , wherein the physicochemical properties include one or more properties selected from the group consisting of: conductivity, ionic strength, ionization state, species identity, pH, absorption spectra, emission spectra, temperature, concentration, effective mobility, and electrophoretic mobility.
8. A method for purification and/or separation of analytes comprising:
performing the method of claim 1 on a sample including analytes.
9. The method of claim 8 , wherein the analytes comprise one or more analytes selected from the group consisting of: DNA, DNA fragments, RNA, RNA fragments, proteins, protein fragments, polypeptides and amino acids.
10. The method of claim 9 , wherein selective isotachophoretic focusing of a selected group of the analytes occurs in a shock wave of the anionic and cationic ITP shock waves.
11. The method of claim 10 , wherein the analytes include one or more species of interest and one or more contaminant species, and wherein the selected group of the analytes includes the one or more species of interest and does not include the one or more contaminant species.
12. The method of claim 11 , wherein the one or more species of interest include DNA species, and wherein the one or more contaminant species include proteins.