Inhibitors of integrin alpha2beta1 based on prolyl diaminopropionic acid scaffold

1. A compound having the formula:

wherein:

X is alkylene, N, O, S, or SO 2 ;

R 0 is alkylene;

R 1 is —NHC(═O)R 2 ;

R 2 is —NH(CH 2 )aryl;

R 3 is H, alkyl, aryl, or aralkyl, or forms a three- to six-membered carbocyclic or heterocyclic ring together with X and the carbon atom to which R 3 and X are both attached;

R 4 and R 5 are each independently H or —CH 3 ;

wherein

the dashed line may represent a double bond;

if R 4 and R 5 are both H, then

X is SO 2 ; or,

X is S and R 3 is not H; or,

X is —CH— and the dashed line represents a double bond;

and,

if X is ethylidene, then one carbon atom of X, R 3 , and the carbon atom to which both are attached form a three- to six-membered carbocyclic or heterocyclic ring, or, X forms a fused bicycle together with Ring A;

or a stereoisomer, partial stereoisomer, pharmaceutically acceptable salt, or N-oxide thereof.

2. A composition comprising a compound according to claim 1 and a pharmaceutically acceptable carrier, diluent, or excipient.

3. A composition comprising a stereochemically enriched mixture of compounds according to claim 2 .

4. The compound according to claim 1 wherein R 1 is —NHC(═O)R 2 , X is S or O, and R 3 is alkyl, aryl, or aralkyl.

5. The compound according to claim 4 wherein X is S and R 4 and R 5 are both H or are both —CH 3 .

6. The compound according to claim 5 wherein said compound is

2-[(3-Benzenesulfonyl-2-methyl-thiazolidine-4-carbonyl)-amino]-3-(3-benzyl-ureido)-propionic acid;

2-[(3-Benzenesulfonyl-2-ethyl-thiazolidine-4-carbonyl)-amino]-3-(3-benzyl-ureido)-propionic acid;

2-[(3-Benzenesulfonyl-2-isopropyl-thiazolidine-4-carbonyl)-amino]-3-(3-benzyl-ureido)-propionic acid;

2-[(3-Benzenesulfonyl-2-tert-butyl-thiazolidine-4-carbonyl)-amino]-3-(3-benzyl-ureido)-propionic acid;

2-[(3-Benzenesulfonyl-2-phenyl-thiazolidine-4-carbonyl)-amino]-3-(3-benzyl-ureido)-propionic acid;

2-[(3-Benzenesulfonyl-2-phenethyl-thiazolidine-4-carbonyl)-amino]-3-(3-benzyl-ureido)-propionic acid;

2-[(3-Benzenesulfonyl-2,5,5-trimethyl-thiazolidine-4-carbonyl)-amino]-3-(3-benzyl-ureido)-propionic acid; or,

2-[(3-Benzenesulfonyl-2-ethyl-5,5-dimethyl-thiazolidine-4-carbonyl)-amino]-3-(3-benzyl-ureido)-propionic acid.

7. The compound according to claim 4 wherein X is O, R 4 is H, R 5 is —CH 3 , and R 3 is alkyl.

8. The compound according to claim 7 wherein the compound is 2-[(3-Benzenesulfonyl-2,5-dimethyl-thiazolidine-4-carbonyl)-amino]-3-(3-benzyl-ureido)-propionic acid.

9. The compound according to claim 1 wherein R 1 is —NHC(═O)R 2 , R 3 , R 4 , and R 5 are each H, and X is SO 2 .

10. The compound according to claim 9 wherein said compound is 2-[(3-Benzenesulfonyl-1,1-dioxo-1λ 6 -thiazolidine-4-carbonyl)-amino]-3-(3-benzyl-ureido)-propionic acid.

11. The compound according to claim 1 wherein X is ethylidene, and:

one carbon atom of X, R 3 , and the carbon atom to which both are attached form a three- to six-membered carbocyclic or heterocyclic ring, or, X forms a fused bicycle together with Ring A.

12. The compound according to claim 11 wherein said compound is

2-[(2-Benzenesulfonyl-1,2,3,4-tetrahydro-isoquinoline-3-carbonyl)-amino]-3-(3-benzyl-ureido)-propionic acid, or

2-[(1-Benzenesulfonyl-1,2,3,4-tetrahydro-quinoline-2-carbonyl)-amino]-3-(3-benzyl-ureido)-propionic acid.

13. The compound according to claim 1 wherein X is —CH—, the dashed line represents a double bond, R 1 is —NHC(═O)R 2 , R 2 is —NH(CH 2 )phenyl, and R 3 , R 4 , and R 5 are each H.

14. The compound according to claim 13 wherein the compound is 2-[(1-Benzenesulfonyl-2,5-dihydro-1H-pyrrole-2-carbonyl)-amino]-3-(3-benzyl-ureido)-propionic acid.

15. A method for treating at least one integrin α2β1-affected disease state or infection comprising the step of administering to a subject in need thereof a composition comprising a therapeutically effective amount of a compound having the formula:

wherein:

X is alkylene, N, O, S, or SO 2 ;

R 0 is alkylene;

R 1 is —NHC(═O)R 2 ;

R 2 is —NH(CH 2 )aryl;

R 3 is H, alkyl, aryl, or aralkyl, or forms a three- to six-membered carbocyclic or heterocyclic ring together with X and the carbon atom to which R 3 and X are both attached;

R 4 and R 5 are each independently H or —CH 3 ;

wherein

the dashed line may represent a double bond;

if R 4 and R 5 are both H, then

X is SO 2 ; or,

X is S and R 3 is not H; or,

X is —CH— and the dashed line represents a double bond;

and,

if X is ethylidene, then one carbon atom of X, R 3 , and the carbon atom to which both are attached form a three- to six-membered carbocyclic or heterocyclic ring, or, X forms a fused bicycle together with Ring A;

or a stereoisomer, partial stereoisomer, pharmaceutically acceptable salt, or N-oxide thereof.

16. The method according to claim 15 wherein the subject is suffering from or susceptible to one or more of acute coronary syndromes, stroke, ischaemic complications of peripheral vascular disease, deep vein thrombosis (DVT), myocardial infarction, coronary artery disease, cerebrovascular disease, peripheral arterial disease, diabetes mellitus, atrial fibrillation, congestive heart failure, pulmonary embolism, and other vascular-related disorders.

17. The method according to claim 15 , wherein the subject is suffering from or susceptible to one or more of human melanoma, hepatocellular carcinoma, breast cancer, lung cancer, ovarian cancer, and other cancers or cancer-related disorders.

18. The method according to claim 15 , wherein the subject is suffering from or susceptible to one or more of rheumatoid arthritis, diabetic retinopathy, and other rheumatoid- or diabetes-related disorders.

19. The method according to claim 15 , wherein the disease state or infection is matrix reorganization-affected, angiogenesis-affected, cell migration-, cell proliferation-, cell colonization-, or metastasis-affected, leukocyte infiltration-affected, edema-affected, or any combination thereof.

20. The method according to claim 15 , where in the subject is suffering from or susceptible to viral infection.

21. The method according to claim 20 , wherein said viral infection is at least partially attributable to human cytomegalovirus (HCMV), rotaviruses, Piconaviridae viruses, or related viruses.

22. The method according to claim 15 wherein said composition additionally comprises a pharmaceutically acceptable carrier, diluent, or excipient.

23. The method according to claim 15 , wherein said composition comprises a stereochemically enriched mixture of compounds of the formula.

24. The method according to claim 15 , wherein said subject is human.