Patent
Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic/transgene junctions within SEQ ID NO: 2 or with genomic/transgene junctions as set forth in SEQ ID NO: 12 and/or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.
1. A method for identifying event DP-073496-4 in a biological sample, comprising
(a) contacting said sample with a first and a second primer, wherein at least one of said first and said second primer targets a DP-073496-4 specific region; and,
(b) amplifying a polynucleotide comprising the DP-073496-4 specific region
wherein the DP-073496-4 specific region comprises a junction sequence selected from the group consisting of SEQ ID NO: 12 and 13.
2. The method of claim 1 , wherein the polynucleotide comprising the DP-073496-4 specific region comprises SEQ ID NO: 14, or 16.
3. The method of claim 1 , wherein the polynucleotide comprising the DP-073496-4 specific region comprises SEQ ID NO: 15, or 17.
4. The method of claim 1 , further comprising detecting the DP-073496-4 specific region.
5. The method of claim 1 , wherein the amplification of a polynucleotide comprising the DP-073496-4 specific region indicates seed purity or seed lot purity.
6. The method of claim 4 , wherein the detection of said DP-073496-4 specific region indicates seed purity or seed lot purity.
7. The method of claim 1 , wherein the first and the second primer can amplify a sequence comprising SEQ ID NO: 12, 13, 14, 15, 16 or 17.