Process for the preparation glatiramer acetate (copolymer-1)
This invention relates to a convenient and improved process for preparation of glatiramer acetate (copolymer-1) of pharmaceutical grade. The process involves polymerizing N-carboxyanhydrides of tyrosine, alanine, y-benzyl glutamate and ε-N-trifluoroacetyllysine in dioxane with diethylamine as initiator to afford protected copolymer-1. Treatment with hydrogen bromide in acetic acid at 35° C. for 3-5 h cleaves benzyl group to produce trifluoroacetyl copolymer-1. The trifluoroacetyl copolymer-1 is washed with an organic solvent to remove reactive benzyl bromide generated during debenzylation. Deprotection with aqueous piperidine, followed by dialysis offers glatiramer acetate (copolymer-1) of Molecular weight 5000-9000 daltons.
1. A process for preparing copolymer-1, comprising:
reacting protected copolymer-1 with hydrobromic acid for 1 to 5 hr at 35-45° C. to form trifluoroacetyl copolymer-1;
washing the trifluoroacetyl copolymer-1 with organic solvent, thus removing benzyl bromide, wherein the organic solvent comprises a hydrocarbon, a halogenated hydrocarbon, an alcohol, an ether, or an ester;
treating the trifluoroacetyl copolymer-1 with aqueous piperidine to form copolymer-1; and
purifying the copolymer-1;
wherein copolymer-1 has a molecular weight of 5,000 to 9,000 Daltons and is glatiramer acetate comprising a mixture of polypeptides composed of glutamic acid, alanine, tyrosine, and lysine in a molar ratio of approximately of 0.141, 0.427, 0.095 and 0.338.
2. The method of claim 1 , comprising reacting the protected copolymer 1 with hydrobromic acid for 3 hr at 35-45° C.
3. The method of claim 1 , wherein the organic solvent comprises diisopropyl ether, diethyl ether, or ethyl acetate.
4. A process for preparing copolymer-1, comprising:
reacting protected copolymer-1 with hydrobromic acid for 1 to 5 hr at 35-45° C. to form trifluoroacetyl copolymer-1;
washing the trifluoroacetyl copolymer-1 with diisopropyl ether, diethyl ether, or ethyl acetate, thus removing benzyl bromide;
treating the trifluoroacetyl copolymer-1 with aqueous piperidine to form copolymer-1; and
purifying the copolymer-1;
wherein copolymer-1 has a molecular weight of 5,000 to 9,000 Daltons and is glatiramer acetate comprising a mixture of polypeptides composed of glutamic acid, alanine, tyrosine, and lysine in a molar ratio of approximately of 0.141, 0.427, 0.095 and 0.338.
5. The method of claim 4 , comprising reacting the protected copolymer 1 with hydrobromic acid for 3 hr at 35-45° C.