Method for producing β-glucanase and xylanase, and liquid culture medium
A method for producing β-glucanase and xylanase which includes the step of culturing a microorganism classified under the genus Trichoderma using a liquid culture medium which contains (a) a pulp derived from paper which has not been subjected to heat treatment nor alkali treatment as a carbon source, and (b) an ammonia nitrogen or an amino nitrogen as a nitrogen source.
1. A method for producing β-glucanase and xylanase comprising the step of culturing Trichoderma reesei in a liquid culture medium containing (a) a pulp derived from paper which has not been subjected to heat treatment nor alkali treatment as a carbon source and (b) an ammonia nitrogen or an amino nitrogen as a nitrogen source, wherein
the concentration of the ammonia nitrogen or amino nitrogen in the liquid culture medium is from 35-660 mM.
2. The method for producing β-glucanase and xylanase according to claim 1 , wherein the concentration of the pulp in the liquid culture medium is not less than 2% W/V.
3. The method for producing β-glucanase and xylanase according to claim 1 , wherein the initial concentration of the pulp in the liquid culture medium is from 2 to 7% W/V.
4. The method for producing β-glucanase and xylanase according to claim 1 , wherein the concentration of the ammonia nitrogen or amino nitrogen in the liquid culture medium is not less than 35 mM.
5. The method for producing β-glucanase and xylanase according to claim 1 , wherein the initial concentration of the ammonia nitrogen or amino nitrogen in the liquid culture medium is from 50-660 mM.
6. The method for producing β-glucanase and xylanase according to claim 1 , wherein the paper is at least one selected from the group consisting of high-quality paper, groundwood paper, copy paper, newspaper and cardboard.
7. The method for producing β-glucanase and xylanase according to claim 1 , wherein the pulp is added to the liquid culture medium in the course of culture.
8. The method for producing β-glucanase and xylanase according to claim 1 , wherein the concentration of the pulp in the liquid culture medium is from 3 to 20% (W/V).