PLGA/HA hydroxyapatite composite bone grafts and method of making
The present invention involves tissue engineering constructs made from a new composite bone graft material made from biocompatible poly(D,L-lactic-co-glycolic acid) (PLGA) and bioceramic particles exposed on its surface using a gas foaming particle leaching (GF/PL) method and infused with collagen. Methods and apparatus for of forming scaffolds are also disclosed.
1. A bone graft biomaterial comprised of a scaffold consisting of a biocompatible polymer and a bioceramic composite in a ratio of about 1:2 to about 2:1 wherein the bioceramic particles are less than 1.0 μm in diameter and wherein the scaffolds contain interconnected pores made via a gas foaming or a particulate leaching process, wherein the bioceramic particles are exposed on the surface of the biomaterial and wherein the biomaterial is impregnated with collagen after the pores are formed by forcing the collagen into the pores by placing the scaffold into a collagen solution and the solution is placed under a vacuum until the temperature is just above the freezing point of the collagen solution, the solution allowed to warm before being subjected to repeated cycles of vacuum and warming.
2. The bone graft material of claim 1 wherein the biocompatible polymer is selected from the group comprising Poly lactic acid (PLA), poly glycolic acid (PGA), Poly lactic co-glycolic acid (PLGA), and copolymers with polyethylene glycol (PEG); polyanhydrides, poly(ortho)esters, polyurethanes, poly(butyric acid), poly(valeric acid), poly(lactide-co-caprolactone) and trimethylene carbonate and combinations and co-polymers thereof.
3. The bone graft material of claim of claim 1 wherein the bioceramic is selected from the group consisting of hydroxyapatite, tricalcium phosphate, bioglass, calcium phosphate or bone or a combination thereof.
4. The bone graft material of claim 3 where in the bioceramic particles are a mixture of hydroxy apatite and TCP in a ratio of about 40:60.
5. The bone graft material of claim 1 wherein the bioceramic particles are from 100 nm to 1000 nm in diameter.
6. The bone graft material of claim 5 wherein the bioceramic particles are from 100 nm to 400 nm in diameter.
7. The bone graft material of claim 1 wherein the biocompatible polymer is poly lactic acid, poly glycolic acid, lactide or caprolactone or copolymers or combinations thereof.
8. The bone graft material of claim 7 comprising two polymers in a ratio from 2:1 to 1:2.
9. The bone graft material of claim 7 where the polymers are lactide and caprolactone in a ratio of about 75:25 by weight.
10. A bone graft biomaterial of claim 1 wherein the bioceramic is TCP coated hydroxyapatite.
11. The bone graft material of claim 1 wherein the ratio of biocompatible polymer to bioceramic ranges from about 80:20 to about 50:50.
12. The bone graft material of claim 11 wherein the ratio of polymer to bioceramic is about 60:40.
13. A bone graft material comprising lactide and caprolactone polymers in a ratio from about 1:2 to about 2:1 which are in a ratio of from about 80:20 to about 50:50 with a bioceramic having a diameter from about 150 microns to about 300 microns comprising hydroxyapatite, TCP, hydroxyapatite TCP or a mixture thereof wherein the scaffold is formed via gas foaming.
14. The bone graft material of claim 13 where in the ratio of lactide to caprolactone is 75:25.
15. The bone graft material of claim 13 wherein the ratio of lactide and caprolactone polymers to bioceramic is about 60:40.