Imaging catheter with integrated reference reflector
In part, the invention relates to a lens assembly. The lens assembly includes a micro-lens; a beam director in optical communication with the micro-lens; and a substantially transparent film. The substantially transparent film is capable of bi-directionally transmitting light, and generating a controlled amount of backscatter. In addition, the film surrounds a portion of the beam director.
1. A method of calibrating an imaging system comprising an interferometer having a reference path length and a sample path length, the method comprising:
generating an image in response to light reflected from a plurality of scattering particles, the scattering particles disposed on or in a substantially transparent curved cover forming a portion of an imaging probe,
adjusting a reference path control motor while analyzing the image;
detecting a pattern in the image, the pattern generated from light scattered from the plurality of scattering particles; and
adjusting the reference path length by an offset measured relative to the pattern such that the reference path length and the sample path length match within an allowed range.
2. The method of claim 1 wherein the step of detecting the pattern further comprises analyzing foreground and background noise levels in the image, wherein the image is a cross-section of a vessel.
3. The method of claim 2 wherein the step of detecting the pattern further comprises forming a binary image.
4. The method of claim 3 wherein the step of detecting the pattern further comprises filtering the binary image with a spatial filter.
5. The method of claim 1 wherein the step of detecting the pattern further comprises emphasizing, using a filter, image content in the image that is concentric or nearly concentric.
6. The method of claim 1 further comprising the step of generating a line for the image in response to light scattered from the scattering particles, the line having a slope.
7. The method of claim 6 wherein the step of detecting the pattern further comprises scoring a plurality of potential patterns for the image based on (i) the brightness of each potential pattern and (ii) the slope.
8. The method of claim 1 further comprising the step of rotating an optical fiber disposed within a sheath, the plurality of scattering particles disposed on or in the sheath, the light reflected from a sample directed to pass through the sheath, wherein the sheath is the substantially transparent curved cover.
9. The method of claim 1 wherein the step of adjusting the reference path control motor comprises:
moving the reference path control motor and
stopping the reference path control motor when the offset has been reached.
10. The method of claim 9 further comprising the step of reactivating the reference path control motor when the sample path length and the reference path length differ by an amount outside an allowed range.
11. The method of claim 1 wherein analyzing the image comprises counting pixels based on their intensity value.
12. The method of claim 11 further comprising the step of storing the pixel counts in memory.
13. The method of claim 1 wherein a sample being imaged is a coronary artery.
14. A method of calibrating an imaging system comprising an interferometer having a reference path length and a sample path length, the method comprising:
generating an image in response to light reflected from a sample and a plurality of scattering particles arranged in a pattern, the scattering particles disposed on or in a substantially transparent curved cover forming a portion of an imaging probe;
scoring a plurality of potential patterns based on brightness of each potential pattern;
selecting the pattern from the plurality of potential patterns based on the scoring; and
adjusting the reference path length by an offset measured relative to the pattern such that the reference path length and the sample path length match at a predetermined location.
15. The method of claim 14 wherein the step of adjusting the reference path length comprises moving a reflector using a reference path control motor connected to the reflector.
16. The method of claim 14 further comprising the step of rotating an optical fiber disposed within a sheath, the pattern disposed on or in the sheath, wherein the sheath is the substantially transparent curved cover.
17. The method of claim 14 wherein the pattern is a ring, a spiral, or an ellipse.
18. The method of claim 14 wherein the sample is a coronary artery.
19. The method of claim 14 further comprising the step of generating a line for the image in response to light scattered from the scattering particles, the line having a slope.
20. The method of claim 19 wherein the step of scoring potential patterns is also based on the slope.
21. The method of claim 17 wherein the wherein the image is a cross-section of a vessel and the pattern is a disposed within the vessel in the image.
22. The method of claim 14 further comprising the steps of
forming a binary image; and
filtering the binary image to identify the plurality of potential patterns.