Anti-Tie2 antibodies and uses thereof
The present invention provides antibodies that bind to Tie2 and methods of using same. According to certain embodiments of the invention, the antibodies are fully human antibodies that bind to human Tie2 and block the interaction between Tie2 and one or more Tie2 ligands such as angiopoietin 1 (Ang1), angiopoietin 2 (Ang2), angiopoietin 3 (Ang3) and/or angiopoietin 4 (Ang4). The antibodies of the invention are useful, inter alia, for the treatment of diseases and disorders associated with one or more Tie2 biological activities including angiogenesis.
1. An isolated antibody or antigen-binding fragment thereof that specifically binds human Tie2 and blocks the interaction between Tie2 and all four of angiopoietin-1 (Ang1), Ang2, Ang3 and Ang4, wherein the antibody or antigen-binding fragment interacts with amino acids 96-106 of SEQ ID NO:7, amino acids 139-152 of SEQ ID NO:7; and amino acids 166-175 of SEQ ID NO:7, as determined by hydrogen/deuterium exchange.
2. The isolated antibody or antigen-binding fragment of claim 1 , wherein the antibody or antigen-binding fragment thereof specifically binds a rodent Tie2 and human Tie2.
3. The isolated antibody or antigen-binding fragment of claim 2 , wherein the rodent Tie2 is mouse Tie2 or rat Tie2.
4. The isolated antibody or antigen-binding fragment of claim 1 , wherein the antibody or antigen-binding fragment thereof specifically binds human, mouse and rat Tie2.
5. The isolated antibody or antigen-binding fragment of claim 1 , wherein the antibody or antigen-binding fragment thereof is a human antibody having the variable regions of an antibody produced from a cell line deposited with the American Type Culture Collection under accession number PTA-12295.
6. A pharmaceutical composition comprising the antibody or antigen-binding fragment of claim 1 and a pharmaceutically acceptable carrier or diluent.
7. An isolated antibody or antigen-binding fragment thereof that binds the same epitope on human Tie2 as the antibody produced from a cell line deposited with the American Type Culture Collection under accession number PTA-12295 or PTA-12296.
8. A pharmaceutical composition comprising the antibody or antigen-binding fragment of claim 7 and a pharmaceutically acceptable carrier or diluent.
9. An isolated antibody or antigen-binding fragment thereof that competes for binding to human Tie2 with an antibody produced from a cell line deposited with the American Type Culture Collection under accession number PTA-12295 or PTA-12296.
10. A pharmaceutical composition comprising the antibody or antigen-binding fragment of claim 9 and a pharmaceutically acceptable carrier or diluent.