Apparatus and method for coupled LC-NMR analysis

1. A device for performing a chromatographic separation and a nuclear magnetic resonance analysis on a sample comprising:

a. a closed chromatographic assembly having an input, an outlet, and chromatographic device said input for receiving one or more samples, said output for discharging one or more separated samples and said chromatographic device for separating said sample to form one or more separated samples having retention time data;

b. conduit means in fluid communication with said closed chromatographic assembly for conveying said one or more separated samples to a nuclear magnetic resonance assembly;

c. a nuclear magnetic resonance assembly for receiving one or more separated samples defined by retention times and producing nuclear magnetic resonance data for said one or more separation samples;

d. control means in signal communication with said closed chromatographic assembly and said nuclear magnetic resonance assembly to receive retention time data and nuclear magnetic resonance data and associating said retention time data and nuclear magnetic resonance data to at least one of said sample and said separated sample;

e. a second detector in fluid communication with said conduit means and in signal communication with said control means, said second detector producing second detector data, said control means associating said second detector data with said retention time data and nuclear magnetic resonance data to at least one of said sample and said separated sample; and

f. a peak detector in fluid communication with said conduit means and in signal communication with said control means, said peak detector producing one or more signals corresponding with an analyte of interest or a potential analyte of interest in a separated sample to isolate said separated sample to form an isolated separated sample and direct said isolated separated sample to at least one of said nuclear magnetic resonance and said second detector.

2. The device of claim 1 wherein said conduit means has trapping means for holding a separated sample to form a held separated sample and placing said held separated sample in said nuclear magnetic resonance assembly.

3. The device of claim 2 wherein said trapping means forms a held separated sample and a passed separated sample, said passed separated sample discharged from said device.

4. The device of claim 3 wherein said trapping means is a trapping column.

5. The device of claim 3 wherein said trapping means is a separated sample loop.

6. The device of claim 2 wherein said trapping means is in fluid communication with nuclear magnetic resonance reagents and said trapping means releases said held separated sample in said nuclear magnetic resonance reagents to form one or more deuterated separated samples for nuclear magnetic resonance analysis.

7. The device of claim 1 wherein said second detector is a mass spectrometer.

8. The device of claim 1 wherein said second detector is a photo diode array detector.

9. The device of claim 1 further comprising valve means in fluid communication with said conduit means, said valve means for receiving said isolated separation sample and forming isolated separated sample aliquots and directing at least one isolated separated sample aliquot to said nuclear magnetic resonance and said second detector such that said isolated separated sample aliquot is associated by control means with nuclear magnetic resonance data, said second detector data and retention time data.