Promoter, promoter control elements, and combinations, and uses thereof
The present invention provides DNA molecules that constitute fragments of the genome of a plant, and polypeptides encoded thereby. The DNA molecules are useful for specifying a gene product in cells, either as a promoter or as a protein coding sequence or as an UTR or as a 3′ termination sequence, and are also useful in controlling the behavior of a gene in the chromosome, in controlling the expression of a gene or as tools for genetic mapping, recognizing or isolating identical or related DNA fragments, or identification of a particular individual organism, or for clustering of a group of organisms with a common trait. One of ordinary skill in the art, having this data, can obtain cloned DNA fragments, synthetic DNA fragments or polypeptides constituting desired sequences by recombinant methodology known in the art or described herein.
1. A vector construct comprising:
a) a first nucleic acid molecule comprising a nucleic acid sequence having at least 99% sequence identity to the nucleotide sequence of SEQ ID NO:1728 or a fragment thereof which comprises the last 1,000 nucleotides from the 3′ end of SEQ ID NO:1728 that functions as a promoter; and
b) a second nucleic acid molecule to be expressed;
wherein said first and second nucleic acid molecules are operably linked and said first nucleic acid molecule is heterologous to said second nucleic acid molecule.
2. A host plant cell comprising an isolated nucleic acid molecule according to claim 1 , wherein said first nucleic acid molecule is flanked by exogenous sequence.
3. A host plant cell comprising the vector construct of claim 1 .
4. A method of introducing an isolated nucleic acid molecule into a host cell comprising:
a) providing an isolated nucleic acid molecule comprising a nucleic acid sequence having at least 99% sequence identity to the nucleotide sequence of SEQ ID NO:1728 or a fragment thereof which comprises the last 1,000 nucleotides from the 3′ end of SEQ ID NO:1728 that functions as a promoter; and
b) contacting said nucleic acid molecule said host cell under conditions that permit insertion of said nucleic acid molecule into said host cell.
5. A plant or cell of a plant which comprises the vector according to claim 1 which is exogenous or heterologous to said plant or plant cell.
6. A plant which has been regenerated from the plant cell according to claim 5 .
7. The vector construct according to claim 1 wherein said first nucleic acid molecule has 1 to 5 nucleotide changes as compared to the nucleotide sequence of SEQ ID NO:1728.
8. The vector construct according to claim 7 wherein said first nucleic acid molecule has one nucleotide sequence change from SEQ ID NO:1728.
9. The vector construct according to claim 1 wherein said first nucleic acid molecule is SEQ ID NO:1728.
10. A method of producing a plant comprising transforming a plant with the vector construct of claim 1 .
11. The method of claim 10 , wherein the first nucleic acid molecule has 1 to 5 nucleotide changes as compared to the nucleotide sequence of SEQ ID NO:1728.
12. The method of claim 10 , wherein the first nucleic acid has one nucleotide change from SEQ ID NO:1728.
13. The method of claim 10 , wherein the first nucleic acid molecule is SEQ ID NO:1728.
14. The method of claim 10 wherein said first nucleic acid molecule has at least 99% sequence identity to the full length nucleotide sequence of SEQ ID NO:1728.