Fast pinhole changer for confocal microscopy or spatial filter
A pinhole changing device for a confocal microscope is detailed herein. Several pinholes of different sizes are contained on a single disk. A precision XY stage is used to switch between the several pinholes. The same device could be used as a spatial filter for a multi-photon microscope. One can also add beam expansion control and spherical aberration correction to the same device with no additional loss.
1. A confocal microscope pinhole changing device comprising:
a light-blocking part located between two lenses with at least two pinhole apertures of at least two different sizes to block out-of-focus light;
an optical system adapted to focus light through at least one of the pinholes; and
a device adapted to move the light-blocking part to select which of the at least one pinhole is in focus of optics.
2. The device of claim 1 , where the light blocking part is a disk or shape adapted to block all light in an optical path except that light which is focused through the pinholes.
3. The device of claim 1 , where the pinholes are arranged in a straight line.
4. The device of claim 1 , where the pinholes are arranged in a two dimensional pattern.
5. The device of claim 1 , where the pinholes are arranged in a three dimensional pattern.
6. The device of claim 1 , further comprising a lens adapted to focus the light.
7. The device of claim 1 , wherein the device adapted to move the light-blocking part is a mechanical stage.
8. The device of claim 7 , wherein the mechanical stage is motorized.
9. The device of claim 8 , wherein the mechanical stage is a piezo driven stage.
10. The device of claim 7 , wherein the mechanical stage has nanometer precision.
11. The device of claim 7 , wherein the mechanical stage can move in two dimensions.
12. The device of claim 7 , wherein the mechanical stage can move in three dimensions.
13. The device of claim 8 , where motion of the mechanical stage is synchronized with one or more external devices, and one of the one or more external devices is a scanner.
14. A pinhole changing system for a confocal microscope comprising:
means for de-scanning a signal associated with the confocal microscope;
a light-blocking part located between two lenses with at least two pinhole apertures of at least two different sizes to block out-of-focus light;
an optical system adapted to focus light through a pinhole; and
means for moving the light-blocking part to select which pinhole is in the focus of optics.
15. The system of claim 14 , wherein the light blocking part is a disk or shape adapted to block all light in an optical path except that light which is focused through the pinhole.
16. The system of claim 14 , wherein the pinholes are arranged in a straight line.
17. The system of claim 14 , wherein the pinholes are arranged in a two dimensional pattern.
18. The system of claim 14 , wherein the pinholes are arranged in a three dimensional pattern.
19. The system of claim 14 , further comprising a lens adapted to focus the light.
20. The system of claim 14 , wherein the means for moving is a mechanical stage.
21. The system of claim 20 , wherein the mechanical stage is motorized.
22. The system of claim 21 , wherein the mechanical stage is a piezo driven stage.
23. The system of claim 20 wherein the mechanical stage has nanometer precision.
24. The system of claim 20 , wherein the mechanical stage can move in two dimensions.
25. The system of claim 20 , where the mechanical stage can move in three dimensions.
26. The system of claim 21 , wherein motion of the mechanical stage is synchronized with one or more external devices, one of the external devices being a scanner.
27. A pinhole changing system for spatial filtering comprising:
a light source;
a light-blocking part located between two lenses with at least two pinhole apertures of at least two different sizes to block out-of-focus light;
an optical system for focusing the light through a pinhole; and
means for moving the light-blocking part to select which pinhole is in the focus of optics.
28. The system of claim 27 , wherein the light blocking part is a disk or shape adapted to block all light in the optical path except that light which is focused through the pinhole.
29. The system of claim 27 , wherein the pinholes are arranged in a straight line.
30. The system of claim 27 , wherein the pinholes are arranged in a two dimensional pattern.
31. The system of claim 27 , wherein the pinholes are arranged in a three dimensional pattern.
32. The system of claim 27 , further comprising one or more lens for focusing the light.
33. The system of claim 27 , wherein the means for moving the pinholes is a mechanical stage and one of:
the mechanical stage is motorized,
the mechanical stage is a piezo driven stage,
the mechanical stage has nanometer precision,
the mechanical stage can move in two dimensions,
the mechanical stage can move in three dimensions,
the motion of the mechanical stage is synchronized with one or more external devices,
the motion of the mechanical stage is synchronized with a scanner,
the optical system further comprises means for re-collimating the light, and
further comprising:
one or more lenses for re-collimating the light, wherein a plurality of lenses can be moved mechanically to provide different amounts of beam expansion or contraction; and
the means for moving is motorized and computer controlled;
the lenses are also moved to provide differing amounts of divergence or convergence to an output beam,
the divergence or convergence is used to correct for spherical aberration in an excitation path of a multi-photon microscope, and
the light source is a pulsed laser used as an excitation source for the multi-photon microscope.