Immunogenic compositions for
The invention includes a GAS antigen, GAS 40, which is particularly suitable for use either alone or in combinations with additional GAS antigens, such as GAS 117, GAS 130, GAS 277, GAS 236, GAS 40, GAS 389, GAS 504, GAS 509, GAS 366, GAS 159, GAS 217, GAS 309, GAS 372, GAS 039, GAS 042, GAS 058, GAS 290, GAS 511, GAS 533, GAS 527, GAS 294, GAS 253, GAS 529, GAS 045, GAS 095, GAS 193, GAS 137, GAS 084, GAS 384, GAS 202, and GAS 057.
1. An immunogenic composition comprising:
(1) a combination of Streptococcus pyogenes (GAS) antigens in substantially pure form, said combination consisting of two to ten GAS antigens, wherein said combination includes:
(A) a Spy0269 antigen which comprises a fragment of 200 or more consecutive amino acids of SEQ ID NO: 1; and
(B) a Spy0416 antigen which comprises a fragment of 200 or more consecutive amino acids of SEQ ID NO: 122; and
(2) an adjuvant, wherein the adjuvant comprises an aluminium salt.
2. The immunogenic composition of claim 1 , wherein the aluminium salt is alum.
3. An immunogenic composition comprising:
(1) a combination of Streptococcus pyogenes (GAS) antigens in substantially pure form, said combination consisting of two to ten GAS antigens, wherein said combination includes:
(A) a Spy0269 antigen which comprises a fragment of 200 or more consecutive amino acids of SEQ ID NO: 1; and
(B) a Spy0416 antigen which comprises a fragment of 200 or more consecutive amino acids of SEQ ID NO: 122; and
(2) an adjuvant, wherein the adjuvant comprises a submicron oil-in water emulsion comprising squalene, polyoxyethylene sorbitan monooleate, and sorbitan trioleate.
4. The immunogenic composition of claim 1 , wherein each of the Spy0269 and Spy0416 antigens is present in the composition at a concentration of at least about 1 μg/ml.
5. The immunogenic composition of claim 3 , wherein each of the Spy0269 and Spy0416 antigens is present in the composition at a concentration of at least about 1 μg/ml.
6. A method of raising an immune response against Streptococcus pyogenes , comprising administering to an animal in need thereof an effective amount of the immunogenic composition of claim 1 .
7. The method of claim 6 , wherein the Spy0269 antigen comprises amino acids 27-849 of SEQ ID NO:1.
8. The method of claim 6 , wherein the Spy0269 antigen lacks amino acids 2-26 of SEQ ID NO:1.
9. The method of claim 7 , wherein the Spy0269 antigen lacks amino acids 2-26 of SEQ ID NO:1.
10. The method of claim 1 , wherein the Spy0269 antigen lacks amino acids 850-873 of SEQ ID NO:1.
11. The method of claim 7 , wherein the Spy0269 antigen lacks amino acids 850-873 of SEQ ID NO:1.
12. The method of claim 8 , wherein the Spy0269 antigen lacks amino acids 850-873 of SEQ ID NO:1.
13. The method of claim 6 , wherein the Spy0269 antigen comprises SEQ ID NO:1.
14. A method of raising an immune response against Streptococcus pyogenes , comprising administering to an animal in need thereof an effective amount of the immunogenic composition of claim 3 .
15. The method of claim 14 , wherein the Spy0269 antigen comprises amino acids 27-849 SEQ ID NO:1.
16. The method of claim 14 , wherein the Spy0269 antigen lacks amino acids 2-26 of SEQ ID NO:1.
17. The method of claim 15 , wherein the Spy0269 antigen lacks amini acids 2-26 of SEQ ID NO:1.
18. The method of claim 14 , wherein the Spy0269 antigen lacks amino acids 850-873 of SEQ ID NO:1.
19. The method of claim 15 , wherein the Spy0269 antigen lacks amino acids 850-873 of SEQ ID NO:1.
20. The method of claim 16 , wherein the Spy0269 antigen lacks amino acids 850-873 SEQ ID NO:1.
21. The method of claim 14 , wherein the Spy0269 antigen comprises SEQ ID NO:1.