Lunasin-containing complex and purification of lunasin from plants
A method of purifying lunasin or a lunasin-containing complex produced in a plant includes providing a plant material from the plant, and isolating the lunasin-containing complex from the plant material, and/or releasing lunasin from the lunasin-containing complex. A composition for treating a cancer or an inflammation-related disease in a subject includes a lunasin-containing complex. A composition for treating a cancer or an inflammation-related disease in a subject includes a lunasin polypeptide and/or a lunasin-containing complex; and curcumin, wherein the combination of the a lunasin polypeptide or lunasin-containing complex and the curcumin has a synergistic effect. A method for treating a cancer or an inflammation-related disease includes administering a composition including a lunasin-containing complex, or a lunasin polypeptide and/or a lunasin-containing complex, and curcumin.
1. A method of purifying lunasin produced in a plant, comprising:
(a) providing a plant material from the plant;
(b) isolating a lunasin-containing complex from the plant material; and
(c) contacting the lunasin-containing complex with another protein with a reducing agent, thereby releasing lunasin from the lunasin-containing complex.
2. The method of claim 1 , wherein the plant is a soybean plant.
3. The method of claim 1 , wherein the plant material is a soy-based material containing a lunasin-containing complex.
4. The method of claim 1 , wherein the plant material is a soybean material obtained as a byproduct of soybean processing.
5. The method of claim 4 , wherein the soybean material is de-fatted soy flour or white flake.
6. The method of claim 1 , wherein the plant material is a hydrated plant material or an extracted plant material.
7. The method of claim 1 , further comprising extracting the plant material.
8. The method of claim 7 , wherein the plant material is extracted using water or an aqueous solution.
9. The method of claim 7 , wherein the plant material is extracted using PBS.
10. The method of claim 1 , wherein the reducing agent is selected form: β-mercaptoethanol (BME), dithiothreitol (DTT), tris(2-carboxyethyl)phosphine (TCEP), and 2-aminoethanethiol.
11. The method of claim 10 , wherein the reducing agent is BME at a concentration of about 100 mM to about 1.5 M.
12. The method of claim 1 , wherein the reducing agent is DTT at a concentration of about 1 mM to about 2 mM.
13. The method of claim 1 , wherein the reducing agent is TCEP at a concentration of about 3 mM to about 100 mM.
14. The method of claim 1 , further comprising purifying the released lunasin.
15. The method of claim 14 , wherein the lunasin is purified using a technique selected from: a size-based filtration technique; a charge-based filtration technique; a hydrophobicity-based filtration technique; or a combination thereof.
16. The method of claim 1 , wherein isolating the lunasin-containing complex comprises using a technique selected from: a size-based filtration technique; a charge-based filtration technique; a hydrophobicity-based filtration technique; or a combination thereof.
17. The method of claim 1 , wherein isolating the lunasin-containing complex comprises subjecting the plant material to ultrafiltration and collecting the permeate.
18. The method of claim 17 , wherein a 50 kD molecular-weight cutoff membrane is used for ultrafiltration.