Bone-repair composition
The present invention relates to a bone-repair composition comprising a micro-pulverized demineralized bone matrix; a nonmicro-pulverized demineralized bone matrix; and a hydrating material. The bone-repair composition of the present invention provides easier injectability and shape-maintenance (handling), and exhibits excellent bone-repairing effects due to a large surface area of the micro-pulverized demineralized bone matrix and an early release of the bone growth factors, compared with conventional compositions comprising the nonmicro-pulverized demineralized bone matrix alone. It also has an advantage of being biocompatible and harmless to the human body because there is no use of synthetic materials.
1. A bone-repair composition comprising a micro-pulverized demineralized bone matrix; a nonmicro-pulverized demineralized bone matrix; and a hydrating material,
wherein the micro-pulverized demineralized bone matrix has a particle size of 0.05 to 250 μm;
wherein the nonmicro-pulverized demineralized bone matrix has a particle size of 250 to 2000 μm; and
wherein the weight ratio of the micro-pulverized demineralized bone matrix:the nonmicro-pulverized demineralized bone matrix:the hydrating material is 0.8:1:3.8.
2. The bone-repair composition of claim 1 , wherein the hydrating material is one or more selected from the group consisting of distilled water, saline solution, concentrated saline solution and ion solution.
3. The bone-repair composition of claim 2 , wherein the hydrating material contains a liquid polyhydroxy compound.
4. The bone-repair composition of claim 3 , wherein the liquid polyhydroxy compound is glycerol or glycerol ester.
5. The bone-repair composition of claim 2 , wherein the hydrating material contains a biocompatible binder.
6. The bone-repair composition of claim 5 , wherein the biocompatible binder is one or more selected from the group consisting of fibrin adhesive, fibrinogen, thrombin, mussel adhesive protein, silk, elastin, collagen, casein, gellatin, albumin, keratin, chitin and chitosan.
7. The bone-repair composition of claim 5 , wherein the biocompatible binder is one or more selected from the group consisting of starch, polylactic acid, polyglycolic acid, polylactic-co-glycolic acid, polydioxanone, polycaprolactone, polycarbonate, polyoxoester, polyamino acid, poly-anhydride, polyhydroxybutylate, polyhydroxyvalerate, poly(propylene glycol-co-fumaric acid), tyrosine-based-polycarbonate, polyvinylpyrrolidone, cellulose, ethyl cellulose and carboxy methyl cellulose.
8. The bone-repair composition of claim 2 , wherein the hydrating material contains one or more selected from the group consisting of antibiotic, vitamins, glucosamine, cytokine and growth factors.
9. The bone-repair composition of claim 1 , further comprising one or more selected from the group consisting of cancellous bone chips, compact bone chips, hydroxyapatite (HA, Ca 10 (PO 4 ) 6 (OH) 2 ), carbonic acid apatite (CA, Ca 10 (PO 4 ) 6 CO 3 ), tricalcium phosphate (TCP, Ca 3 (PO 4 ) 2 ), calcium pyrophosphate (Ca 2 P 2 O 7 ), anorganic bone, dental tooth enamel, aragonite, calcite, nacre, graphite, pyrolytic carbon, calcium-silicate-based bioglass, Al 2 O 3 and ZrO 2 .
10. The bone-repair composition of claim 1 , wherein the micro-pulverized demineralized bone matrix and the nonmicro-pulverized demineralized bone matrix are those which have been treated with an enzyme.
11. The bone-repair composition of claim 10 , wherein the enzyme used for the treatment is one or more selected from the group consisting of pepsin, trypsin, chymotrypsin and papain.
12. The bone-repair composition of claim 1 , wherein the micro-pulverized demineralized bone matrix and the nonmicro-pulverized demineralized bone matrix are those which have been prepared under refrigeration at 1 to 10° C.
13. The bone-repair composition of claim 1 , wherein the amount of residual calcium in the micro-pulverized demineralized bone matrix and the nonmicro-pulverized demineralized bone matrix is 8% or less.