Translation enhancer-element dependent vector systems
A translation enhancer-driven positive feedback vector system is disclosed which is designed to facilitate identification of a Translational Enhancer Element (TEE) and to provide a means for overexpression of gene products. The system exploits both transcriptional and translational approaches to control the expression levels of genes and/or gene products. Methods are also disclosed for screening libraries of random nucleotide sequences to identify translational elements and for overproduction of proteins, which have uses in both research and industrial environments.
1. A polycistronic vector suitable for use in a mammalian host cell comprising:
a) an isolated first polynucleotide construct comprising in an operable manner in a 5′ to 3′ direction: (i) two or more first transcriptional elements comprising a TATA box promoter element comprising nucleotides 148-153 of SEQ ID NO:1 in combination with four copies of the GAL4 upstream activating sequence (UAS) comprising nucleotides 19-35, 38-54, 57-73, and 76-92 of SEQ ID NO:1, (ii) at least one first translational enhancer elements (TEEs) comprising one N18 random oligonucleotide sequence comprising nucleotides 190-207 of SEQ ID NO:1, and (iii) a first cistron comprising nucleotides 214-897 of SEQ ID NO:1 and encoding a first gene product, wherein the first gene product is a yeast regulatory protein GAL4/viral fusion protein 16 (GAL4/VP16), and wherein said N18 is operably linked to the first cistron and increases the amount of GAL4/viral protein induced per unit mRNA as related to the absence of said N18 random oligonucleotide sequence;
b) an isolated second polynucleotide construct comprising in an operable manner in a 5′ to 3′ direction: (i) at least one second transcriptional element comprising a TATA box promoter element comprising nucleotides 1347-1352 of SEQ ID NO:1 in combination with four copies of the GAL4/UAS comprising nucleotides 1224-1240, 1243-1259, 1262-1278, and 1281-1297 of SEQ ID NO:1, (ii) at least a second TEEs comprising internal ribosome entry sites (IRES) elements having translational regulatory activity, and (iii) a second cistron encoding a second gene product, wherein the second TEEs is operably linked to the second cistron; and
c) an isolated third polynucleotide construct comprising in an operable manner in a 5′ to 3′ direction: (i) at least one third transcriptional element, (ii) at least a third TEEs comprising internal ribosome entry sites (IRES) elements having translational regulatory activity, and (iii) a third cistron encoding a third gene product, wherein said third TEEs is operable linked to the third cistron, wherein said third gene product is selected from the group consisting of a Rotavirus NSP3 protein, L-proteinase, and proteinase 2A,
wherein the first, second, and third constructs are functionally linked, wherein the expression of the GAL4/VP16 results in binding to GAL4 upstream activating sequence (UAS) sites in the transcriptional element promoters of the first and second polynucleotide sequences and increases their transcription, wherein the resulting level of gene product expressed from the second polynucleotide construct is enhanced in the presence of the first and second TEEs, and wherein expression of the third gene product increases the translation of the first and second cistron by decreasing the competition arising from cellular mRNAs in a mammalian host cell.
2. The vector of claim 1 , wherein the IRES element in the second or third TEEs is a Gtx sequence.
3. The vector of claim 1 , wherein the second cistron encodes a reporter protein, therapeutic protein, an enzyme, an antigen, an antibody, or a structural protein.
4. The vector of claim 3 , wherein the reporter protein is GFP, luciferase, b-galactosidase, b-glucuronidase, alkaline phosphatase, chloramphenicol acetyltransferase, ECFP, EGFP, or EYFP.